C2888

Acetylcholinesterase from Electrophorus electricus (electric eel)

Type V-S, lyophilized powder, ≥1,000 units/mg protein

• Synonyme(s) : AChE, Acetylcholine acetylhydrolase, Cholinesterase, Acetyl
• CAS Number: 9000-81-1
• Numéro CE : 3.1.1.7 (BRENDA, IUBMB)
• Numéro CE : 232-559-3
• Numéro MDL: MFCD00081268
• Code UNSPSC : 12352204
• Nomenclature NACRES : NA.32

Propriétés

• Source biologique: Electrophorus electricus
• Niveau de qualité: 200
• Type: Type V-S
• Forme: lyophilized powder
• Activité spécifique: ≥1,000 units/mg protein
• Poids mol.: 280 kDa
• Composition: Protein, ≥45% biuret
• pl: ~5.3
• Solubilité: Tris buffer: 1 mg/mL (0.02 M Tris buffer, pH 7.5)
• Température de stockage: −20°C

Description

Description générale

Molecular Weight: 280 kDa
Isoelectric Point: 5.5
Extinction Coefficient: E^1% = 18.0 (280 nm)

Acetylcholinesterase from Electrophorus electricus is a tetramer composed of 4 equal subunits of 70 kDa each. Each subunit contains one active site. The enzyme is a glycoprotein containing hexosamines.

Application

Acetylcholinesterase from Electrophorus electricus (electric eel) has been used:

• in acetylcholinesterase activity assay
• to study its effects on Xenopus embryonic hippocampal cells
• to study the effect of acetylcholine inhibitors on development and plasticity

Actions biochimiques/physiologiques

Acetylcholinesterase (AChE) exhibits its activity against acetylcholine in the neuromuscular junctions and synaptic cleft of cholinergic neurons. It participates in the nitric oxide signaling pathway.

Major degradative enzyme for acetylcholine in vivo. Converts acetylcholine + H₂O to choline + acetic acid.

Attention

For dilute solutions (<1 mg/ml), add 1 mg/ml BSA to stabilize. Solutions containing at least 1 mg protein/ml in dilute neutral phosphate buffer are stable at least 6 months refrigerated.

Définition de l'unité

One unit will hydrolyze 1.0 μmole of acetylcholine to choline and acetate per min at pH 8.0 at 37 °C.

Forme physique

Lyophilized powder containing Tris buffer salts

Remarque sur l'analyse

The activity obtained using acetylcholine as substrate is 30-100 times that obtained with butyrylcholine, using acetylcholinesterase from electric eel.

Autres remarques

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)