M9004

Malic Dehydrogenase from bovine heart

ammonium sulfate suspension, 2000-4000 units/mg protein (modified Warburg-Christian)

• Synonyme(s) : L-Malate:NAD⁺ oxidoreductase, MDH
• Numéro CE : 1.1.1.37 (BRENDA, IUBMB)
• Numéro CE : 232-622-5
• Numéro MDL: MFCD00131567
• Code UNSPSC : 12352204
• Nomenclature NACRES : NA.54

Propriétés

• Source biologique: bovine heart
• Niveau de qualité: 200
• Forme: ammonium sulfate suspension
• Activité spécifique: 2000-4000 units/mg protein (modified Warburg-Christian)
• Poids mol.: 35 kDa
• Impuretés: ≤0.01% Glutamic-Oxalacetic Transaminase; ≤0.01% Glutamic-Pyruvic Transaminase
• Température de stockage: 2-8°C

Description

Description générale

Malic Dehydrogenase from bovine heart contains a histidine residue at the NAD-binding active site which is critical for activity. When this histidine is mutated a loss in activity is observed.

Malic dehydrogenase is a cytoplasmic isozyme and an important catalyst in the tricarboxylic acid cycle.

Application

Malic dehydrogenase has been used in a study to assess a flow injection system for on-line monitoring of fumaric acid in biological processes. It has also been used in a study to investigate a root-knot nematode parasitizing peanut in Texas.

Définition de l'unité

One unit will convert 1.0 μmole of oxalacetate and β-NADH to _L-malate and β-NAD per min at pH 7.5 at 25 °C.

Forme physique

Suspension in 3 M (NH₄)₂SO₄ - 0.01 M KH₂PO₄ solution, pH 7.3

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)