C6423

α-Chymotrypsin from bovine pancreas

suitable for protein sequencing, salt-free, lyophilized powder

• CAS Number: 9004-07-3
• Numéro CE : 3.4.21.1 (BRENDA, IUBMB)
• Numéro CE : 232-671-2
• Numéro MDL: MFCD00130481
• Code UNSPSC : 12352204
• eCl@ss : 42010112
• Nomenclature NACRES : NA.26

Propriétés

• Qualité: Proteomics Grade
• Niveau de qualité: 200
• Forme: salt-free, lyophilized powder
• Poids mol.: 25 kDa
• Adéquation: suitable for protein sequencing
• Numéro d'accès UniProt: P00767
• Température de stockage: 2-8°C
• Informations sur le gène: cow ... CTRB1(618826)

Description

Description générale

α-Chymotrypsin from bovine pancreas (bovine pancreatic α-chymotrypsin, CHT) is an enzyme protein. The influence of varying concentrations of organic solvents like ethanol, 1,4-dioxane and acetonitrile on CHT has been reported.

Chymotrypsin (Chy) is a serine protease. It corresponds to a molecular weight of 25.7 kDa and is widely used in pharmaceutical industry. It is synthesized in pancreas from chymotrypsinogen and require calcium for this conversion.

Application

α-Chymotrypsin from bovine pancreas is used for the following applications:

• Protein Identification by mass spectrometry (MS)
• The isolation and characterization of myosin heavy chains
• Toxin preparation
• The incubation of infected and uninfected cells for analysis of cellular proteins by SDS-PAGE

α-Chymotrypsin from bovine pancreas (BPC) may be used as a catalyst in the preparation of tetrahydroquinoline derivatives by Povarov reaction.

Actions biochimiques/physiologiques

α-Chymotrypsin from bovine pancreas is stabilized by Ca²⁺ and catalyzes the hydrolysis of peptide bonds in particular the amino acids tyrosine, phenylalanine, tryptophan, and leucine at their C-terminal side. α-Chymotrypsin is inhibited by Cu²⁺ and Hg.

A serine protease that hydrolyzes peptide bonds with aromatic or large hydrophobic side chains (Tyr, Trp, Phe, Met, Leu) on the carboxyl end of the bond.

Définition de l'unité

One unit will hydrolyze 1.0 μmole of BTEE per min at pH 7.8 at 25 °C.

Autres remarques

View more information on chymotrypsin at www.sigma-aldrich.com/enzymeexplorer

Informations sur la sécurité

• Pictogrammes: GHS08,GHS07,GHS09
• Mention d'avertissement: Danger
• Mentions de danger: H302,H315,H319,H334,H335,H400
• Conseils de prudence: P261 - P264 - P273 - P301 + P312 - P302 + P352 - P305 + P351 + P338
• Classification des risques: Acute Tox. 4 Oral - Aquatic Acute 1 - Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• Organes cibles: Respiratory system
• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 1
• Équipement de protection individuelle: dust mask type N95 (US), Eyeshields, Faceshields, Gloves