D1821

DAF-FM

≥98% (HPLC)

• Synonyme(s) : 4-Amino-5-methylamino-2′,7′-difluorescein, Diaminofluorescein-FM
• Formule empirique (notation de Hill) : C₂₁H₁₄F₂N₂O₅
• CAS Number: 254109-20-1
• Poids moléculaire : 412.34
• Numéro MDL: MFCD29073049
• Code UNSPSC : 12352204
• ID de substance PubChem : 329798711
• Nomenclature NACRES : NA.77

Propriétés

• Niveau de qualité: 100
• Essai: ≥98% (HPLC)
• Forme: powder
• Conditions de stockage: desiccated
• Couleur: white to brown
• Solubilité: DMSO: >1 mg/mL
• Conditions d'expédition: wet ice
• Température de stockage: −20°C
• Chaîne SMILES: CNc1ccc2c(c1N)C(=O)OC23c4cc(F)c(O)cc4Oc5cc(O)c(F)cc35
• InChI: 1S/C21H14F2N2O5/c1-25-13-3-2-8-18(19(13)24)20(28)30-21(8)9-4-11(22)14(26)6-16(9)29-17-7-15(27)12(23)5-10(17)21/h2-7,25-27H,24H2,1H3
• Clé InChI: DIJCILWNOLHJCG-UHFFFAOYSA-N

Description

Application

DAF-FM has been used in the visualization of nitric oxide (NO) in root sections of wheat seedlings. It has also been used to label neural crest (NC) cells.

Actions biochimiques/physiologiques

DAF-FM is a highly sensitive, photo-stable fluorescent probe for the detection of nitric oxide (NO).

DAF-FM is a highly sensitive, photo-stable fluorescent probe for the detection of nitric oxide (NO). DAF-2 is the original compound and is widely used, but DAF-FM is more sensitive to nitric oxide (NO) compared with DAF-2. DAF-FM is more photo-stable and less pH sensitive than DAF-2. DAF-FM is not quite as cell-permeable as the diacetate derivative, DAF-FM, but it does not need to be activated by cytosolic enzymes.

Attention

hygroscopic

Notes préparatoires

Color variation within the range from white to brown does not change performance.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable