D3812

DirectLoad^™ 50 bp DNA Step Ladder

ready-to-use marker for DNA electrophoresis

• Synonyme(s) : DirectLoad^™ 50 bp DNA Marker, Ready-to-use 50 bp DNA Marker, Ready-to-use DirectLoad^™ 50 bp DNA Marker, Ready-to-use DirectLoad^™ 50 bp DNA Step Ladder
• Code UNSPSC : 41105335
• Nomenclature NACRES : NA.25

Propriétés

• Niveau de qualité: 200
• Forme: (liquid)
• Utilisation: sufficient for 100 loads
• Adéquation: suitable for electrophoresis (DNA)
• Température de stockage: −20°C

Description

Description générale

Sigma′s DirectLoad^™ 50 bp DNA Step Ladder contains 17 blunt-ended fragments consisting of 50 bp repeats from 50 to 500 bp, 100 bp repeats from 600 to 900 bp, and 1 kb repeats from 1 to 3 kb. Ten ul of the ladder can be loaded directly in a single lane on an agarose or polyacrylamide gel.

Application

DirectLoad^™ 50 bp DNA Step Ladder has been used for cell lysis and genomic cleavage detection assay.

Suitable for size determination of PCR-generated DNA fragments with either agarose or polyacrylamide gels.

Caractéristiques et avantages

• Ready-to-load
• Easy-to-use
• Popular band sizes

Composants

Sigma′s DirectLoad^™ 50 bp DNA Step Ladder is provided in a gel-loading solution of 5% glycerol, 4.2 mM EDTA, 0.083% orange G and 0.0125% xylene cyanol.

Autres remarques

For optimal resolution, the recommended agarose gel concentration is 2.0%.

Informations légales

DirectLoad is a trademark of Sigma-Aldrich Co. LLC

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable