D4764

Deoxyribonucleic acid from calf thymus

Genomic, unsheared

• Synonyme(s) : double-stranded template DNA, Calf Thymus DNA, DNA from calf thymus, Thymonucleic acid
• CAS Number: 91080-16-9
• Numéro CE : 293-507-3
• Numéro MDL: MFCD00151698
• Code UNSPSC : 41106310
• eCl@ss : 32160414
• Nomenclature NACRES : NA.52

Propriétés

• Qualité: Molecular Biology
• Niveau de qualité: 200
• Forme: lyophilized powder
• Poids mol.: >20 kb
• Température de stockage: 2-8°C

Description

Description générale

High quality double-stranded template DNA isolated from the thymus of male and female calves.

Spécificité

42% GC; T_m = 87 °C.
% GC is the percentage of G/C base pairs. T_m is the temperature at the midpoint of the thermal denaturation profile.

Application

Calf thymus DNA is exceptionally useful as a substrate for DNA polymerase assays, in the amplification of very long fragments and as a carrier DNA for precipitations.

Calf thymus DNA is exceptionally useful as a substrate for DNA polymerase assays, in the amplification of very long fragments and as a carrier DNA for precipitations. It was used as a standard for DNA quantification by fluorescent assay. It was used as reference to determine the DNA content in cartilage tissue digest samples.

Caractéristiques et avantages

• No nuclease activity detected after 16 h at 37 °C incubation
• High quality template DNA

Notes préparatoires

Purified by anion exchange chromatography, dialyzed against a solution of 1 mM sodium chloride, 1 mM EDTA and 1 mM Trizma HCl, pH 7.5 and lyophilized at a concentration of ~2 A₂₆₀ units per mL. ~20 A₂₆₀ units per mg DNA.

Reconstitution

Dissolve the DNA gently rocking overnight to a final concentration of 1mg/ml in molecular biology grade water.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves