D8899

Deoxyribonucleic acid, single stranded from calf thymus

lyophilized powder

• Synonyme(s) : DNA single stranded
• CAS Number: 91080-16-9
• Numéro CE : 293-507-3
• Numéro MDL: MFCD00163436
• Code UNSPSC : 41106310
• eCl@ss : 32160414
• Nomenclature NACRES : NA.52

Propriétés

• Qualité: Molecular Biology
• Niveau de qualité: 200
• Forme: lyophilized powder
• Poids mol.: ~50 kb
• Composition: ssDNA, ≥65%
• Température de stockage: −20°C

Description

Description générale

High quality single-stranded template DNA isolated from the thymus of male and female calves.

Application

Calf thymus DNA is exceptionally useful as a substrate for DNA polymerase assays, in the amplification of very long fragments and as a carrier DNA for precipitations.

Calf thymus DNA is exceptionally useful as a substrate for DNA polymerase assays, in the amplification of very long fragments and as a carrier DNA for precipitations. It was used as a standard for DNA quantification by fluorescent assay and in DNA-binding assay.

Caractéristiques et avantages

• Activated
• No nuclease activity detected after 16 h at 37 °C incubation
• High quality template DNA

Quantité

One mg DNA is approx. 25 A₂₆₀ units.

Notes préparatoires

Prepared by a modification of the method of Alberts and Herrick from calf thymus DNA.

Reconstitution

Dissolve the DNA gently rocking overnight to a final concentration of 1mg/ml in molecular biology grade water.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves