D6063

REDTaq^® SuperPak^™ DNA Polymerase

Taq for routine PCR with inert dye; with 10X buffer & dNTP mix

• Synonyme(s) : DNA polymerase with dNTP, DNA polymerase with loading dye, Taq DNA polymerase
• Code UNSPSC : 41106300
• Nomenclature NACRES : NA.55

Propriétés

• Niveau de qualité: 200
• Forme: liquid
• Utilisation: sufficient for 250 reactions
• Caractéristiques: dNTPs included; hotstart: no
• Concentration: 1 unit/μL
• Technique(s): PCR: suitable
• Couleur: red
• Entrée: purified DNA
• Adéquation: suitable for PCR
• Conditions d'expédition: wet ice
• Température de stockage: −20°C

Description

Description générale

REDTaq^® SuperPak^™ DNA Polymerase is Sigma′s Taq DNA Polymerase mixed with an inert red dye. REDTaq^® SuperPak^™ DNA Polymerase is a convenient package, that includes all the necessary components for a PCR reaction except primers, DNA template and water. It consists of Sigma′s high quality REDTaq DNA polymerase, 10 mM ultrapure deoxynucleotide mix and 10´ PCR reaction buffer. The dye provides quick recognition of reactions to which enzyme has been added as well as visual confirmation of complete mixing.

Application

REDTaq^® SuperPak^™ DNA Polymerase has been used in:

• reverse transcriptase-polymerase chain reaction (RT-PCR)
• nested PCR
• routine PCR amplification

Caractéristiques et avantages

• Contains inert dye that enables visual confirmation of enzyme addition and direct loading onto agarose gel without the need for an additional loading dye
• Suitable for nested PCR
• Contains ultrapure 10mM dNTP mix in a separate vial

Informations légales

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: US 8,404,464 and US 7,972,828. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims.

REDTaq is a registered trademark of Merck KGaA, Darmstadt, Germany

SuperPak is a trademark of Sigma-Aldrich Co. LLC

Composants de kit également disponibles séparément

• P2192 10X PCR Buffer, Optimized for routine PCR with MgCl₂ included
• D7295 Deoxynucleotide Mix, 10 mM, Molecular Biology Reagent

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable