P2192

10X PCR Buffer

Optimized for routine PCR with MgCl₂ included

• Synonyme(s) : PCR Buffer Solution, PCR Reaction Buffer
• Code UNSPSC : 41106306
• Nomenclature NACRES : NA.52

Propriétés

• Niveau de qualité: 300
• Forme: liquid
• Conditionnement: vial of 1.5 mL
• Couleur: colorless
• Application(s): agriculture
• Activité étrangère: DNase, RNase, none detected
• Température de stockage: −20°C

Description

Application

10X PCR Buffer has been used as a component of the PCR mixture:

• for the amplification of viral DNA
• to amplify genomic DNA to study the methylenetetrahydrofolate reductase (MTHFR) gene mutations in attention deficit hyperactivity disorder (ADHD) individuals
• to amplify 16S-23S rRNA gene internal transcribed spacer (ITS) from Bacillus sp.

Caractéristiques et avantages

• Standalone buffer
• Compatible with JumpStart^™ Taq DNA Polymerase (D9307), Taq DNA Polymerase from Thermus aquaticus (D1806), and REDTaq^® Genomic DNA Polymerase (D8312)

Notes préparatoires

Composition of the 10× buffer: 100 mM Tris-HCl, pH 8.3 at 25°C; 500 mM KCl; 15 mM MgCl₂; 0.01% gelatin

Informations légales

JumpStart is a trademark of Sigma-Aldrich Co. LLC

REDTaq is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves