D9780

Lambda DNA Hind III Digest

for DNA electrophoresis

• Synonyme(s) : Hind III digested bacteria phage Lambda DNA
• Code UNSPSC : 41141827
• Nomenclature NACRES : NA.25

Propriétés

• Qualité: Molecular Biology
• Niveau de qualité: 200
• Forme: liquid
• Utilisation: 100 uses (0.1 mg size); 1000 uses (1 mg size); 20 uses (20 μg size); 500 uses (0.5 mg size)
• Adéquation: suitable for electrophoresis (DNA)
• Conditions d'expédition: dry ice
• Température de stockage: −20°C

Description

Description générale

Sigma′s Lambda DNA Hind III Digest contains 8 fragments from 125 – 23,130 bp. Ten ul of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel.

Application

Suitable for size determination of dsDNA by electrophoresis with either agarose or polyacrylamide gels.

Composants

Sigma′s Lambda DNA Hind III Digest is provided in a solution of 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA.

Autres remarques

Contains 8 fragments, 125–23,130 bp

For optimal resolution, the recommended agarose gel concentration is 0.75%.

Notes préparatoires

Bacteriophage lambda DNA was digested with EcoR I and Hind III to completion, then purified by phenol extraction.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable