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ER0100

Sigma-Aldrich

Endoplasmic Reticulum Isolation Kit

isolation of intact ER from mammalian soft tissues and cultured cells

Synonyme(s) :

ER isolation kit

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About This Item

Code UNSPSC :
12352200
Nomenclature NACRES :
NA.32

Niveau de qualité

200

Technique(s)

fractionation: suitable

Température de stockage

2-8°C

Catégories apparentées

Description générale

The endoplasmic reticulum (ER) is a network of interconnected tubules, vesicles, and sacs. It plays a role in specialized cellular functions including protein synthesis, sequestering of calcium, production of steroids, storage and production of glycogen, and insertion of membrane proteins.

Application

The Endoplasmic Reticulum Isolation Kit contains all the reagents required for ER preparations with various degrees of purity: crude ER (microsomes), Ca2+ precipitated rough ER (RER) enriched microsomes, and density gradient purified rough ER (RER) and smooth ER (SER). The ER isolation process can be monitored by measuring the activity of NADPH cytochrome c reductase using the Cytochrome c Reductase Assay Kit, (Cat. No. CY0100). The enzyme is an ER membrane protein that is commonly used as an ER marker. The isolated ER can be used for studying the cytochrome P450 system and xenobiotic metabolism, for studying lipid metabolism, and for the recovery of ER membrane and lumenal proteins. The kit was tested with rat liver, kidney, and brain; mouse liver; rabbit liver, kidney, spleen, heart, and brain; and with Jurkat and HeLa cell lines.
The Endoplasmic Reticulum Isolation Kit is used for the efficient enrichment of functional endoplasmic reticulum (ER) from mammalian tissue or cell culture. The isolated ER are useful for protein synthesis, degradation and metabolic pathway analysis. Metabolic abnormalities of ER include carnitine acetyltransferase deficiency in children, cystic fibrosis and many more ER protein retention disorders. Suitable for up to 50 g of tissue or ~20 mL of packed tissue.

Caractéristiques et avantages

  • Specially formulated extraction reagents for research scale applications - save time and minimize waste
  • Includes a calcium chloride solution - for quick and simple precipitation of rough ER without need for ultracentrifugation
  • Produces functional, intact organelles - resulting ER are suitable for functional studies, lipid metabolism, and protein profiling
  • Compatible with products for structure confirmation - easily confirm intactness with companion kit test for cytochrome C reductase (CY0100)
  • Can be used to isolate ER from soft animal tissues and cultured cells

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

08168

Timestrip Plus™ 8 °C

Pictogrammes

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GHS07

Mention d'avertissement

Warning

Mentions de danger

H319

Classification des risques

Eye Irrit. 2

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 1

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Certificats d'analyse (COA)

Lot/Batch Number
079M4125V
11190115
038M4161V
11171024
086M4108V

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