NUC101

Nuclei Isolation Kit: Nuclei EZ Prep

sufficient for 25 nuclei preparations (~1-10×10⁷ cells/preparation)

• Synonyme(s) : Rapid mammalian nuclei isolation kit
• Code UNSPSC : 12352207
• Nomenclature NACRES : NA.32

Propriétés

• Utilisation: sufficient for 25 nuclei preparations (~1-10×10⁷ cells/preparation)
• Niveau de qualité: 200
• Durée de conservation: 1 yr at 2‑8 °C
• Conditionnement: pkg of 1 kit
• Application(s): cell analysis
• Activité étrangère: nuclease and protease, free
• Conditions d'expédition: wet ice
• Température de stockage: 2-8°C

Description

Description générale

The Nuclei EZ Prep Kit was developed for the rapid isolation of nuclei from mammalian cells. The kit provides a high yield of nuclei from commonly used cell types.

Application

Suitable as a source of nuclear components, to produce nuclei for in vitro apoptosis assays, and for functional studies.

Actions biochimiques/physiologiques

The protocol provides a high yield of nuclei from commonly used mammalian cells, including both adherent (e.g., HEK293 and COS7) and non-adherent (e.g., Jurkat and HFN7.1) cell lines and peripheral blood mononuclear cells (PBMCs). The preparations are suitable for many cell biology applications, e.g., as a source of nuclear components such as chromatin, genomic DNA, histones and nuclear RNA/RNP, produces nuclei for in vitro apoptosis assays, and functional studies such as examination of the transcriptional status of cells.

Autres remarques

All procedures should be carried out on ice or 2-8°C.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable