F8646

Anti-Mouse IgG (Fc specific) F(ab′)₂ fragment−FITC antibody produced in goat

affinity isolated antibody, buffered aqueous solution

• Numéro MDL: MFCD00162628
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.46

Propriétés

• Source biologique: goat
• Niveau de qualité: 200
• Conjugué: FITC conjugate
• Forme d'anticorps: affinity isolated antibody
• Type de produit anticorps: secondary antibodies
• Clone: polyclonal
• Forme: buffered aqueous solution
• Conditions de stockage: protect from light
• Technique(s): immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:320
• Température de stockage: −20°C
• Modification post-traductionnelle de la cible: unmodified

Description

Description générale

IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections
Anti-Mouse IgG (Fc specific) F(ab′)₂ fragment−FITC antibody is specific for the Fc fragment of mouse IgG. No cross reactivity is observed with the Fab fragment of mouse IgG. Purified antibody is conjugated to fluorescein isothiocyanate (FITC) in an alkaline reaction and then purified to remove unbound FITC.

Spécificité

Binds mouse IgG; does not bind other mouse Igs.

Immunogène

Purified mouse IgG

Application

Anti-Mouse IgG (Fc specific) F(ab′)₂ fragment−FITC antibody may be used for immunohistochemistry at a working dilution of 1:320 using formalin-fixed, paraffin-embedded sections of human tonsil. Antibody dilution of 1:200 was used to label sections of human brainstems and cervical spinal cords with primary antibody against human Bcl-2 protein. The antibody was also used to label erythrocytes for photon reassignment microscopy and for FACS using melanoma cells.

Autres remarques

Antibody adsorbed with bovine, equine, and human serum proteins.

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Notes préparatoires

Adsorbed to reduce background with bovine, equine, or human samples.
Useful when trying to avoid background staining due to the presence of Fc receptors.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: dust mask type N95 (US), Eyeshields, Gloves