G1549

PNGase F from Elizabethkingia meningoseptica

ready-to-use solution, recombinant, expressed in E. coli

• Synonyme(s) : N-Glycosidase F, PNGase F, Peptide N-glycosidase
• CAS Number: 83534-39-8
• Numéro CE : 3.5.1.52 (BRENDA, IUBMB)
• Numéro MDL: MFCD00131222
• Code UNSPSC : 12352204
• Nomenclature NACRES : NA.54

Propriétés

• Produit recombinant: expressed in E. coli
• Niveau de qualité: 200
• Conjugué: (N-linked)
• Qualité: Proteomics Grade
• Forme: ready-to-use solution
• Activité spécifique: ≥1000 U/mg
• Durée de conservation: ≥1 yr at -20 °C
• Poids mol.: ~36 kDa
• Conditions d'expédition: wet ice
• Température de stockage: −20°C

Description

Application

Recombinant PNGase F has been purified by affinity chromatography and dialyzed into a 50% glycerol solution with 10 mM potassium phoosphate pH 7.5 to produce a stable product. The product contains low levels of buffer salts. This highly purified material can be used for preparative deglycosylation or for analytical applications in gel, in solution, or on blot membranes. The enzyme can be removed from preparative operations by utilizing its C-terminal 6x histidine fusion tag. PNGase F from Elizabethkingia meningoseptica has been used in deglycosylation assay in human plasma samples and in deglycosylation of chondroitin sulfate proteoglycan.

Produit utilisé pour la déglycosylation des protéines.

Actions biochimiques/physiologiques

PNGase F from Elizabethkingia meningoseptica has glycan-binding catalytic domain and a bowl-like domain at the N-terminus. It cleaves an entire glycan from a glycoprotein provided the glycosylated asparagine moiety is substituted on its amino and carboxyl terminus with a polypeptide chain. It is cost-effectively produced on a large scale in prokaryotic hosts and requires divalent zinc ions for its enzymatic activity.

Clive un glycane entier d′une glycoprotéine, à condition que le fragment d′asparagine glycosylé soit substitué sur ses extrémités amino et carboxyle avec une chaîne polypeptidique.

Définition de l'unité

One unit will catalyze the release of N-linked oligosaccharides from 1 nanomole of denatured ribonuclease B in one minute at 37°C at pH 7.5 monitored by SDS-PAGE. One Sigma unit of PNGase F activity is equal to 1 IUB milliunit.

Forme physique

Supplied as 300 Units/mL enzyme in 50% (v/v) glycerol and 50% (v/v) 20 mM Potassium Phosphate, pH 7.5.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable