G1924

L-Glutamate Oxidase from Streptomyces sp.

recombinant, expressed in E. coli

• Synonyme(s) : L-Glutamate:oxygen oxidoreductase (deaminating)
• CAS Number: 39346-34-4
• Numéro MDL: MFCD00131459
• Code UNSPSC : 12352204
• Nomenclature NACRES : NA.54

Propriétés

• Produit recombinant: expressed in E. coli
• Niveau de qualité: 200
• Forme: solid
• Activité spécifique: ≥5 units/mg protein
• Poids mol.: 120 kDa
• Température de stockage: −20°C

Description

Description générale

L-Glutamate Oxidase (LGOX) from Streptomyces sp is secreted extracellularly in the wheat bran pre cultivation medium. It corresponds to a molecular weight of 120 kDa with monomer subunit of 61 kDa. The structure of LGOX comprises of hexamer and has flavin adenine dinucleotide prosthetic group.

This product has a 6xHistidine tag with a thrombin cleavage site.

Application

L-Glutamate Oxidase from Streptomyces sp may be used:

• as a reference enzyme in hydrogelpoly(carbamoylsulphonate) based immobilization for biosensor development
• as a component of pencil graphite electrode for biosensing L-glutamate

Actions biochimiques/physiologiques

L-Glutamate Oxidase (LGOX) catalyzes the oxidative deamination of L-glutamate to α-ketoglutarate, ammonia and hydrogen peroxide. LGOX is industrially produced and is exploited majorly as a biosensor for L-glutamate detection and in the L-glutamate kits.

Définition de l'unité

One unit will form 1.0 micromole of alpha-ketoglutaric acid from L-glutamic acid per minute at pH 7.4 at 30 deg C.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable