G5885

Glucose-6-phosphate Dehydrogenase from Leuconostoc mesenteroides

lyophilized powder, >= 550 units/mg protein (biuret)

• Synonyme(s) : G-6-P-DH
• CAS Number: 9001-40-5
• Numéro CE : 1.1.1.49 (BRENDA, IUBMB)
• Numéro CE : 232-602-6
• Numéro MDL: MFCD00081656
• Code UNSPSC : 12352204
• Nomenclature NACRES : NA.54

Propriétés

• Source biologique: bacterial (Leuconostoc mesenteroides)
• Niveau de qualité: 300
• Type: Type XXIV
• Forme: lyophilized powder
• Activité spécifique: >= 550 units/mg protein (biuret)
• Poids mol.: 128 kDa
• Composition: Protein, 15-40% biuret
• Application(s): agriculture
• Activité étrangère: 6-Phosphogluconic dehydrogenase, hexokinase, NADH oxidase and NADPH oxidase ≤0.005%; PGI ≤0.01%
• Température de stockage: 2-8°C

Description

Description générale

Glucose-6-Phophate Dehydrogenase (G-6-PDH) comprises His-Asp catalytic dyad and exists as a homodimer. Structurally, G-6-PDH encompasses a Rossmann dinucleotide binding fold in the coenzyme binding domain region. It also harbors a large β + α domain and has a unique aspartate residue at position 374.

Application

Glucose-6-phosphate Dehydrogenase from Leuconostoc mesenteroides has been used along with hexokinase in the determination of glucose from mice liver samples.

Actions biochimiques/physiologiques

Glucose-6-Phophate Dehydrogenase (G-6-PDH) can utilize either nicotinamide adenine dinucleotide phosphate (NADP⁺) or NAD⁺ as coenzyme making it crucial for bacterial metabolism.

Glucose-6-phosphate dehydrogenase (G6PD) catalyzes the conversion of glucose-6-phosphate to 6-phosphogluconolacetone as the first step in the pentose phosphate pathway.

Définition de l'unité

One unit will oxidize 1.0 μmole of D-glucose 6-phosphate to 6-phospho-D-gluconate per min in the presence of NAD at pH 7.8 at 30 °C.

Forme physique

Lyophilized powder containing Ficoll and Tris buffer salts

Informations sur la sécurité

• Pictogrammes: GHS08
• Mention d'avertissement: Danger
• Mentions de danger: H334
• Conseils de prudence: P261 - P284 - P501
• Classification des risques: Resp. Sens. 1
• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)