G8404

Glucose-6-phosphate Dehydrogenase from Leuconostoc mesenteroides

recombinant, expressed in E. coli, ammonium sulfate suspension, ≥550 units/mg protein (biuret)

• Synonyme(s) : Entner-Doudoroff enzyme, G6PD, G6PDH, NADP glucose 6-phosphate dehydrogenase, G-6-P-DH
• CAS Number: 9001-40-5
• Numéro CE : 1.1.1.49 (BRENDA, IUBMB)
• Numéro MDL: MFCD00081656
• Code UNSPSC : 12352204
• Nomenclature NACRES : NA.54

Propriétés

• Source biologique: bacterial (Leuconostoc mesenteroides)
• Niveau de qualité: 300
• Produit recombinant: expressed in E. coli
• Forme: ammonium sulfate suspension
• Activité spécifique: ≥550 units/mg protein (biuret)
• Poids mol.: 128 kDa
• Conditions de stockage: (Tightly closed)
• Technique(s): cell culture | mammalian: suitable
• Numéro d'accès UniProt: UPI0021A4D550
• Activité étrangère: creatine phosphokinase, glutathione reductase, myokinase, NADH oxidase, NADPH oxidase, phosphoglucomutase, 6-phosphogluconic dehydrogenase, phosphoglucose isomerase, lactic dehydrogenase, hexokinase ≤0.01%
• Température de stockage: 2-8°C

Description

Description générale

Glucose 6-phosphate dehydrogenase (G-6-P-DH) is a key regulatory enzyme in the first step of the pentose phosphate pathway. G-6-P-DH is a glycoprotein with a molecular mass of 128 kDa (gel filtration).G6PD is a dimer and consists of a single active site at each subunit.

Research area: Cell Signaling

Application

Glucose-6-phosphate dehydrogenase has been used:

• to test ketose reductase activity in developing maize endosperm.
• to determine the levels of mannose in coronary heart disease patient-derived serum
• to study its activity on extracellular polymeric substance (EPS) extract to determine cell lysis through sonication
• to determine the glucose uptake in cultured human muscle satellite cells

Actions biochimiques/physiologiques

G6PD provides all cells with reducing power as nicotinamide adenine dinucleotide phosphate (NADPH) which enables the cells to balance oxidative stress. Mutations in the G6PD gene are associated with X-linked G6PD deficiency, a hereditary genetic defect such as neonatal jaundice and acute hemolytic anemia.

Glucose-6-phosphate dehydrogenase (G6PD) catalyzes the conversion of glucose-6-phosphate to 6-phosphogluconolacetone as the first step in the pentose phosphate pathway.

Définition de l'unité

One unit will oxidize 1.0 μmole of D-glucose 6-phosphate to 6-phospho-D-gluconate per min in the presence of NAD at pH 7.8 at 30 °C.

Forme physique

Suspension in 3.2 M (NH₄)₂SO₄ containing 50 mM Tris and 1 mM MgCl₂, pH 7.5

Autres remarques

The volume is lot specific and can be calculated using the data:
For example, lot 0000114274
Units per mg Protein = 846
Mg Protein per mL - 9
Units per mL = 7614
G8404-1000U = approx 132 ul

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type ABEK (EN14387) respirator filter