G9270

γ-Glutamyltranspeptidase from equine kidney

Type VI, 5-12 units/mg solid

• Synonyme(s) : γ-GT, γ-Glutamyltransferase, (5-Glutamyl)peptide:amino-acid 5-glutamyltransferase, GGTP
• CAS Number: 9046-27-9
• Numéro CE : 2.3.2.2 (BRENDA, IUBMB)
• Numéro CE : 232-931-5
• Numéro MDL: MFCD00131126
• Code UNSPSC : 12352204
• Nomenclature NACRES : NA.54

Propriétés

• Source biologique: equine kidney
• Niveau de qualité: 200
• Type: Type VI
• Forme: solid
• Activité spécifique: 5-12 units/mg solid
• Température de stockage: −20°C

Description

Description générale

Gamma-Glutamyltranspeptidase is a heterodimeric enzyme and belongs to the superfamily of N-terminal nucleophile hydrolases.

Application

Gamma-glutamyltranspeptidase from equine kidney has been used:

• to identify the formation of 4-S-Cysteinyltetrodotoxin in the liver of Fugu pardalis
• as a standard to determine the activity of gamma-glutamyltransferase from human B-cell lymphoma cell lysates
• to hydrolyze isopeptides to study its effects on tubulin aggregation

Actions biochimiques/physiologiques

Gamma-Glutamyltranspeptidase participates in glutathione metabolism and it catalyzes the cleavage of gamma-glutamyl compounds, such as glutathione. It also plays a role in the transfer of gamma-glutamyl moiety to amino acids and peptides.

Qualité

Crude

Définition de l'unité

One unit will liberate 1.0 μmole of p-nitroaniline from L-γ-glutamyl-p-nitroanilide per min at pH 8.5 at 25°C.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)