H8509

β-Hydroxybutyrate Dehydrogenase from Rhodopseudomonas sphaeroides

Type V, lyophilized powder, 250-750 units/mg protein

• Synonyme(s) : (R)-3-Hydroxybutanoate:NAD⁺ oxidoreductase, 3-HBDH
• CAS Number: 9028-38-0
• Numéro CE : 1.1.1.30 (BRENDA, IUBMB)
• Numéro CE : 232-830-6
• Numéro MDL: MFCD00130633
• Code UNSPSC : 12352204
• Nomenclature NACRES : NA.54

Propriétés

• Type: Type V
• Niveau de qualité: 200
• Forme: lyophilized powder
• Activité spécifique: 250-750 units/mg protein
• Produit purifié par: chromatography
• Activité étrangère: lactate dehydrogenase ≤0.05%; malate dehydrogenase ≤0.1%
• Température de stockage: −20°C

Description

Application

Suitable for the determination of acetoacetate and D(-)-3-hydroxybutyrate by the method of Williamson, D. H., and Mellanby, J., Methods of Enzymatic Analysis, Bergmeyer, H., ed., 2nd edition, 4, 1836 (1974).

Actions biochimiques/physiologiques

In mammalian systems, β-hydroxybutyrate dehydrogenase is localized on the inner mitochondrial membrane and requires phosphatidyl choline for activity. In contrast, the enzyme from Rhodopseudomonas is a soluble cytosolic enzyme that does not require a phospholipid allosteric activator. The enzyme is required for the utilization of ketone bodies as a source of metabolic energy. It catalyzes the oxidation of 3-hydroxybutyrate to acetoacetate, the first step in the conversion of ketone bodies to citric acid, which is then further metabolized via the tricarboxylic acid cycle (Krebs cycle).

Définition de l'unité

One unit will oxidize 1.0 μmole of D-β-hydroxybutyrate to acetoacetate per min at pH 7.8 at 37 °C.

Forme physique

Lyophilized powder containing Tris buffer salts

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)