H9377

Hypoxanthine

≥99.0%

• Synonyme(s) : 6-Hydroxypurine
• Formule empirique (notation de Hill) : C₅H₄N₄O
• CAS Number: 68-94-0
• Poids moléculaire : 136.11
• Beilstein: 5811
• Numéro CE : 200-697-3
• Numéro MDL: MFCD00005725
• Code UNSPSC : 41106305
• ID de substance PubChem : 24895850
• Nomenclature NACRES : NA.51

Propriétés

• Source biologique: synthetic (organic)
• Niveau de qualité: 300
• Essai: ≥99.0%
• Forme: powder
• Pf: >300 °C (lit.)
• Solubilité: formic acid: water (2:1): 50 mg/mL, clear to slightly hazy, colorless to yellow
• Chaîne SMILES: O=C1NC=Nc2nc[nH]c12
• InChI: 1S/C5H4N4O/c10-5-3-4(7-1-6-3)8-2-9-5/h1-2H,(H2,6,7,8,9,10)
• Clé InChI: FDGQSTZJBFJUBT-UHFFFAOYSA-N

Description

Application

Hypoxanthine is a nutrient additive for a variety of cell culture applications involving bacterial, parasite (Plasmodium falciparum) and animal cells. Hypoxanthine is a component of selection media used in hybridoma technologies.

Hypoxanthine has been used:

• to gavage mice and to evaluate the activity of xanthine oxidase (XO) in pasteurized whole milk in vivo in potassium oxonate (PO) -induced mouse model of hyperuricemia
• as a supplement in Iscove′s modified Dulbecco′s medium (IMDM) medium to cultivate in-vitro cell line (EL-1 cells)
• as a pure standard for the quantification of hypoxanthine in vitreous fluid sample using high-performance liquid chromatography-ultra-violet (HPLC-UV) for the estimation of post-mortem interval

Actions biochimiques/physiologiques

Hypoxanthine acts as an intermediate in purine metabolism. It elicits a vital role as a 5′-base of the anticodon in a few transfer-RNAs. Though similar to guanine (Gua), it is devoid of the exocyclic amino group at C(2) position. It may be used as a substrate to study the specificity and kinetics of hypoxanthine-guanine phosphoribosyl transferases.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)