I4401

Interferon-αA/D human

≥95%, recombinant, expressed in E. coli, buffered aqueous solution, suitable for cell culture

• Synonyme(s) : IFN-αA/D
• Numéro MDL: MFCD01635116
• Code UNSPSC : 12352202
• Nomenclature NACRES : NA.32

Propriétés

• Source biologique: human
• Niveau de qualité: 200
• Produit recombinant: expressed in E. coli
• Essai: ≥95%
• Forme: buffered aqueous solution
• Poids mol.: 19 kDa
• Conditionnement: pkg of 100K units
• Technique(s): cell culture | mammalian: suitable
• Impuretés: endotoxin, tested
• Couleur: colorless
• Numéro d'accès UniProt: P01563
• Conditions d'expédition: dry ice
• Température de stockage: −70°C
• Informations sur le gène: human ... IFNA2(3440)

Description

Application

Interferon-αA/D human has been used:

• for identification of coronavirus replicase inhibitors
• in combination with ACH-806 [1-(4-pentyloxy-3-trifluoromethylphenyl)-3-(pyridine-3-carbonyl)thiourea] to study the effect on hepatitis C virus (HCV) replication
• in combination with vitamin D to check reduction in HCV protein production in cell culture

Actions biochimiques/physiologiques

Interferon-αA/D (Bg/II), also known as Universal Type I Interferon, is an α interferon hybrid that crosses the species barrier. The Bg/II denotes the restriction enzyme site from which this hybrid is constructed from recombinant human interferons αA and αD. Interferon-αA/D has been proven to be active on many mammalian cells. It can substitute for human, monkey, mouse, bovine, rat, cat, dog, rabbit, sheep, goat, horse, pig or hamster type I interferon (α, β, ω and τ). Type I Interferons are a closely related family of 165-172 amino acid proteins that are produced by leukocytes (α subtypes), fibroblasts (β subtypes), lymphocytes (ω subtypes), and ruminant embryos (τ subtypes). This cytokine has anti-viral, anti-proliferative, immunoregulatory, and proinflammatory activities.

This cytokine has anti-viral, anti-proliferative, immunoregulatory, and proinflammatory activities.

Forme physique

Solution in phosphate buffered saline containing 0.1% bovine serum albumin

Remarque sur l'analyse

The biological activity is determined in the cytopathic effect inhibition assay using MDBK cells with vesicular stomatitis virus (VSV). A cytopathic effect of 50% is produced with 1 unit/ml interferon. The units are determined with respect to NIH international standard reference for human interferonαA.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves