M0268

Milieu essentiel minimum d′Eagle

With Earle′s salts and L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

• Synonyme(s) : MEM
• Code UNSPSC : 12352207
• Nomenclature NACRES : NA.75

Propriétés

• Niveau de qualité: 500
• Forme: powder
• Technique(s): cell culture | mammalian: suitable
• Composants: HEPES: no; phenol red: yes; Earle’s salts (5% CO₂): yes; NaHCO₃: no; L-glutamine: yes; sodium pyruvate: no
• Conditions d'expédition: ambient
• Température de stockage: 2-8°C

Description

Description générale

Minimum Essential Medium Eagle (MEM) is one of the most widely used synthetic cell culture media. MEM has been used for culturing several cells grown in monolayers. It is commonly used to grow attached cell lines, such as fibroblasts, in the presence of fetal bovine sera (FBS), calf, or horse sera.

Application

Minimum Essential Medium Eagle has been used:

• in the isolation and culture of primary hepatocytes
• to culture the human endometrial carcinoma cells Ishikawa (ATTC) and L-Wnt-3a cells (ATCC) that secrete Wnt family member 3a (Wnt-3a) ligand
• to culture the AN3CA (endometrial cancer cell line) cells, and HEC1-B human (endometrial adenocarcinoma cell line) cells

Caractéristiques et avantages

• Minimum Essential Medium Eagle (MEM) contains higher concentrations of amino acids to mimic the protein composition of cultured mammalian cells.
• The formulation has been altered by optional elimination of calcium to enable the growth of cells in suspension culture.

Quantité

Formulated to contain 9.5 grams of powder per liter of medium.

Reconstitution

Supplement with 2.2 g/L sodium bicarbonate.

Autres remarques

Essentially the same as the original formula, except it is provided as a powder.

Informations sur la sécurité

• Code de la classe de stockage: 13 - Non Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable