M5755

MOPS-EDTA-Sodium Acetate (MESA)

powder for RNA electrophoresis buffers

• Synonyme(s) : MESA Buffer
• Code UNSPSC : 12161700
• Nomenclature NACRES : NA.25

Propriétés

• Qualité: Molecular Biology
• Niveau de qualité: 200
• Gamme de produits: BioReagent
• Forme: powder
• Technique(s): electrophoresis: suitable
• pH: 6.8-7.2(reconstituted per directions)
• Solubilité: H₂O: soluble, faintly yellow
• Activité étrangère: DNAses, RNases, NICKases, endonucleases, and exonucleases, none detected
• Température de stockage: room temp

Description

Description générale

MOPS – EDTA – Sodium Acetate (MESA) is the most commonly used running buffer for RNA electrophoresis prior to Northern blotting procedures. Applied voltage of 5 V/cm is recommended for maximum resolution.

Application

Suitable for use when making formaldehyde-agarose gels and associated running buffer for RNA electrophoresis.

Notes préparatoires

1x MESA buffer contains:

• 40 mM MOPS
• 10 mM sodium acetate
• 1 mM EDTA (pH 8.3)

Reconstitution

Dissolve entire contents of bottle in a final volume of 1L molecular biology grade water. This produces a 10x MESA stock solution that can be further diluted as needed.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)