N1665

5′-Nucleotidase human

recombinant, expressed in CHO cells, vial of 6-12 μg

• Synonyme(s) : CD73, NT5E, ecto-5′-nucleotidase
• CAS Number: 9027-73-0
• Numéro CE : 3.1.3.5 (BRENDA, IUBMB)
• Numéro MDL: MFCD00130311
• Code UNSPSC : 12352204
• Nomenclature NACRES : NA.54

Propriétés

• Produit recombinant: expressed in CHO cells
• Niveau de qualité: 200
• Description: contains a C-terminal 6-His tag
• Essai: ≥90% (SDS-PAGE)
• Forme: solution
• enzyme activity: ≥15 units/mg protein
• Poids mol.: ~61 kDa by SDS-PAGE (reducing)
• Conditionnement: vial of 6-12 μg
• Impuretés: ≤1.0 EU/μg endotoxin
• Numéro d'accès NCBI: NP_002517
• Conditions d'expédition: dry ice
• Température de stockage: −70°C
• Informations sur le gène: human ... NT5E(4907)

Description

Description générale

5′-Nucleotidase is an extracellular enzyme found in a wide variety of mammalian cells. It helps to accelerate the hydrolysis reaction of phosphate group from 5′ nucleotides, resulting in corresponding nucleosides.

Actions biochimiques/physiologiques

5′-Nucleotidase has various clinical significances. It is a key molecule in the regulation of cancer cells proliferation, migration and invasion in vitro tumor angiogenesis, and tumor immune escape in vivo. Due to this important role, the enzyme is a potential target for cancer research. It is also involved in salvage of extracellular nucleotides and plays a key role in the control of tissue homeostasis.

5′-nucleotidase, also known as CD73, is an extracellular enzyme that converts nucleoside-5′−monophosphates to nucleosides with a substrate preference of AMP. Native 5′-nucleotidase is a GPI-anchored protein whose exporession is upregulated by hypoxia. 5′-nucleotidase has many functions in vivo including the generation of extracellular adenosine.

Définition de l'unité

One unit will hydrolyze 1.0 μmole of inorganic phosphorus from adenosine 5′-monophosphate per min at pH 9.0 at 37 °C.

Forme physique

Supplied as a solution containing Tris, NaCl, CaCl_2, and 20% glycerol.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable