P3243

Phosphodiesterase I from Crotalus adamanteus venom

vial of ≥100 units, Purified

• Synonyme(s) : 5′-Exonuclease, Oligonucleate 5′-nucleotidohydrolase
• CAS Number: 9025-82-5
• Numéro CE : 3.1.4.1 (BRENDA, IUBMB)
• Numéro CE : 232-806-5
• Numéro MDL: MFCD00131883
• Code UNSPSC : 12352204
• Nomenclature NACRES : NA.54

Propriétés

• Source biologique: Crotalus adamanteus venom
• Niveau de qualité: 200
• Forme: solid
• Qualité: Purified
• Activité spécifique: ≥20.0 unit/mg solid
• Conditionnement: vial of ≥100 units
• Température de stockage: −20°C

Description

Application

Phosphodiesterase (PDE) is any enzyme that is used to breaks phosphodiester bonds. It is a membrane-bound glycoprotein that is used to catalyze the hydrolysis of various nucleotide polyphosphates. Phosphodiesterase I is used in phosphodiesterase activation assays to hydrolyze AMP. Product P3243 is from Crotalus adamanteus venom and is purified. Product P3243 has been used to hydrolyze tRNA with wyosine derivatives into mononucleosides.

Actions biochimiques/physiologiques

Phosphodiesterase I breaks phosphodiester bonds and catalyzes the hydrolysis of various nucleotide polyphosphates. Phosphodiesterase I is released from eucaryotic plasma membranes by phosphatidylinositol-specific phospholipase C.

Définition de l'unité

One Unit hydrolyzes one μmole of p-nitrophenyl thymidine-5-phosphate per minute at 25 °C, pH 8.9

Notes préparatoires

Purified via the method of Williams, et al. and further treated to inactivate contaminating 5′-nucleotidase activity.

Reconstitution

For testing purposes, PDE I is reconstituted in cold deionized water at 0.1 - 0,2 un/mL.

Informations sur la sécurité

• Pictogrammes: GHS08
• Mention d'avertissement: Danger
• Mentions de danger: H334
• Conseils de prudence: P261 - P284 - P501
• Classification des risques: Resp. Sens. 1
• Code de la classe de stockage: 13 - Non Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable