P7306

Polyethylene glycol/ dimethyl sulfoxide solution

Hybri-Max^™, average mol wt 1,450, 50 % (w/v), 0.2 μm filtered, BioReagent, suitable for hybridoma

• Synonyme(s) : PEG/DMSO Solution, Polyethylene Glycol Solution, Solution of PEG and DMSO
• Numéro MDL: MFCD00081839
• Code UNSPSC : 12161902
• ID de substance PubChem : 24898839
• Nomenclature NACRES : NA.75

Propriétés

• Qualité: Hybri-Max^™
• Stérilité: 0.2 μm filtered
• Gamme de produits: BioReagent
• Forme: solution
• Poids mol.: average mol wt 1,450
• Concentration: 50 % (w/v)
• Technique(s): cell culture | hybridoma: suitable
• Impuretés: endotoxin, tested
• Température de stockage: 2-8°C
• Chaîne SMILES: C(CO)O
• InChI: 1S/C2H6O2/c3-1-2-4/h3-4H,1-2H2
• Clé InChI: LYCAIKOWRPUZTN-UHFFFAOYSA-N

Description

Application

PEG is used as a fusogen to obtain hybridomas for monoclonal antibody production. Induces cell hybridization.


Recommended for use in a normal fusion protocol requiring 50% PEG and 10% DMSO.

Conditionnement

Packaged in sealed ampules under nitrogen.

Autres remarques

Contains 50% (w/v) polyethylene glycol (Av. Mol. Wt. 1450) and 10% DMSO (v/v) in DPBS without Calcium.

Reconstitution

Solution is ready-to-use. If a less concentrated solution is desired, dilute with sterile DPBS without calcium (D5773). Some precipitate may appear after being exposed to cooler, but should disappear as the solution warms. Solution may be frozen if desired but should first be aliquotted to avoid repeated freeze/thaw cycles.

Informations légales

Hybri-Max is a trademark of Sigma-Aldrich Co. LLC

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable