R2520

Ribonuclease inhibitor human

Inhibits ribonucleases during transcription and translation experiments

• Synonyme(s) : RNase Inhibitor, Ribonuclease Inhibitor
• Numéro MDL: MFCD00132187
• Code UNSPSC : 12352200
• Nomenclature NACRES : NA.52

Propriétés

• Source biologique: human placenta
• Niveau de qualité: 200
• Qualité: Molecular Biology
• Forme: buffered aqueous solution
• Poids mol.: ~50 kDa
• Concentration: 30,000-50,000 units/mL
• Conditions d'expédition: dry ice
• Température de stockage: −20°C

Description

Description générale

Ribonuclease inhibitor works to inhibit RNase activity by forming a tight, non-covalent 1:1 complex. It is human placenta. It inhibits RNases A, B, and C. It will not inhibit RNases H, 1, T1, S1 Nuclease, SP6, T7 RNA Polymerase, T3 RNA Polymerase, AMV Reverse Transcriptase, M-MLV Reverse Transcriptase, or Taq Polymerase. The inhibitor can be removed by phenol extraction or by heating to 65°C for 10 minutes.

Application

Suitable for use in

• in vitro inhibition of ribonucleases
• cDNA synthesis
• RT-PCR
• in vitro transcription and translation

Useful for in vitro inhibition of ribonucleases, including procedures like cDNA synthesis, RT-PCR, and in vitro transcription and translation.

Actions biochimiques/physiologiques

Inhibits RNase by forming a tight, non-covalent 1:1 complex.

Inhibits RNase by forming a tight, non-covalent 1:1 complex. Also inhibits angiogenin, a neovascularization promoter which is homologous with RNase A.

Composants

Ribonuclease Inhibitor is provided as a solution in 20 mM HEPES-KOH( pH 7.6), 50 mM KCl, 8 mM DTT and 50% glycerol.

Autres remarques

This inhibitor of RNase activity is isolated from human placenta and supplied as a buffered aqueous glycerol solution.

Solution in 50% glycerol, 20 mM HEPES-KOH, pH 7.6, 50 mM KCl and 8 mM DTT

Définition de l'unité

One unit will reduce the activity of 5 ng of ribonuclease A by 50% in a cytidine 2′:3′-cyclic monophosphate system.

Forme physique

Solution in 50% glycerol, 20 mM HEPES-KOH, pH 7.6, 50 mM KCl and 8 mM DTT

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter