R4408

Chloramphenicol

Ready Made Solution, 100 mg/mL in ethanol: isopropanol (95:5), ≥98% (HPLC)

• Synonyme(s) : D-(-)-threo-2,2-Dichloro-N-[β-hydroxy-α−(hydroxymethyl)-β-(4-nitrophenyl)ethyl]acetamide, D-(-)-threo-2-Dichloroacetamido-1-(4-nitrophenyl)-1,3-propanediol, D-threo-2,2-Dichloro-N-[β-hydroxy-α-(hydroxymethyl)-4-nitrophenethyl]acetamide
• Code UNSPSC : 51102831
• Nomenclature NACRES : NA.85

Propriétés

• Niveau de qualité: 200
• Essai: ≥98% (HPLC)
• Forme: solution
• Concentration: 100 mg/mL in ethanol: isopropanol (95:5)
• Spectre d'activité de l'antibiotique: Gram-negative bacteria; Gram-positive bacteria
• Mode d’action: protein synthesis | interferes
• Conditions d'expédition: dry ice
• Température de stockage: −20°C

Description

Application

Chloramphenicol is a synthetic antibiotic, isolated from strains of Streptomyces venezuelae. It is often used for bacterial selection in molecular biology applications at 10-20 μg/mL and as a selection agent for transformed cells containing chloramphenicol reistance genes.

Actions biochimiques/physiologiques

Mode of Action: Chloramphenicol inhibits bacterial protein synthesis by blocking the peptidyl transferase step by binding to the 50S ribosomal subunit and preventing attachment of aminoacyl tRNA to the ribosome. It also inhibits mitochondrial and chloroplast protein synthesis and ribosomal formation of (p)ppGpp, de-pressing rRNA transcription.

Mode of Resistance: Use of chloramphenicol acetyltransferase will acetylate the product and inactivate it.

Antimicrobial Spectrum: This is a broad spectrum antibiotic against gram-positive and gram-negative bacteria, and is used mainly for ophthalmic and veterinary purposes.

Conditionnement

10ml

Notes préparatoires

This product is a ready made solution of 100 mg/mL in ethanol:isopropanol (95:5). It is shipped in dry ice and stored at -20°C.

Autres remarques

Keep container tightly closed in a dry and well-ventilated place. Containers which are opened must be carefully resealed and kept upright to prevent leakage.Storage class (TRGS 510): Flammable liquids")

Informations sur la sécurité

• Pictogrammes: GHS02,GHS08,GHS05
• Mention d'avertissement: Danger
• Mentions de danger: H225,H318,H351,H361fd
• Conseils de prudence: P202 - P210 - P233 - P280 - P305 + P351 + P338 - P308 + P313
• Classification des risques: Carc. 2 - Eye Dam. 1 - Flam. Liq. 2 - Repr. 2
• Code de la classe de stockage: 3 - Flammable liquids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): 57.2 °F - closed cup
• Point d'éclair (°C): 14.0 °C - closed cup