R8000

D-Ribulose 1,5-Diphosphate Carboxylase from spinach

partially purified powder, 0.01-0.1 unit/mg solid

• Synonyme(s) : 3-Phospho-D-glycerate carboxy-lyase(dimerizing), Rubisco
• CAS Number: 9027-23-0
• Numéro CE : 4.1.1.39 (BRENDA, IUBMB)
• Numéro MDL: MFCD00130908
• Code UNSPSC : 12352204
• Nomenclature NACRES : NA.54

Propriétés

• Source biologique: spinach
• Niveau de qualité: 200
• Forme: partially purified powder
• Activité spécifique: 0.01-0.1 unit/mg solid
• Poids mol.: 557 kDa
• Température de stockage: −20°C

Description

Description générale

D-Ribulose 1,5-Diphosphate Carboxylase (RUBISCO) amounts to 50% of the total spinach leaves associated soluble protein.

Exists as a 557 kDa hexadecamer composed of eight heavy chains each with a molecular weight of approximately 56 kDa and eight light chains of molecular weight 14 kDa. Each molecule contains one magnesium ion.
pH optimum: ~7.9.
K_M for CO₂: ~0.45 mM.
Ribulose diphosphate becomes inhibitory at concentrations exceeding 0.7 mM. Orthophosphate and ammonium sulfate are competitive inhibitors. 3-Phosphoglycerate is a noncompetitive inhibitor.

Application

D-Ribulose 1,5-Diphosphate Carboxylase from spinach has been used:

• as a test protein in pepsin digestion studies
• as an innocuous or non-hazardous protein sample to test its effect on human intestinal epithelial cell lines
• in isothermal titration calorimetry (ITC), and radiolabeled binding assays with abscisic acid

Actions biochimiques/physiologiques

D-Ribulose 1,5-Diphosphate Carboxylase (RUBISCO) depends on Rubisco activase and chaperones for activation. It participates in plant photorespiration events by catalyzing the carboxylation and oxygenation of ribulose-1,5-bisphosphate. Abscisic acid inhibits the carboxylation activity of Rubisco.

Définition de l'unité

One unit will convert 1.0 μmole of D-RuDP and CO₂ to 2.0 μmoles of D-3-phosphoglycerate per min at pH 7.8 at 25°C.

Informations sur la sécurité

• Pictogrammes: GHS08
• Mention d'avertissement: Danger
• Mentions de danger: H334
• Conseils de prudence: P261 - P284 - P501
• Classification des risques: Resp. Sens. 1
• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)