S1797

SOC Medium

For use in transformation

• Synonyme(s) : Super Optimal broth
• Code UNSPSC : 41106200
• Nomenclature NACRES : NA.85

Propriétés

• Qualité: Molecular Biology
• Niveau de qualité: 500
• Stérilité: 0.2 μm filtered
• Forme: liquid
• Composition: Dextrose, 3.603 g/L ; KCl, 0.186 g/L ; MgSO₄, 4.8 g/L ; Tryptone, 20 g/L ; Yeast extract, 5 g/L
• Technique(s): microbiological culture: suitable
• Application(s): agriculture
• Température de stockage: 2-8°C
• Adéquation: nonselective for Escherichia coli; nonselective for coliforms

Description

Description générale

Super optimal medium with catabolic repressor (SOC) medium is a glucose-rich microbial growth medium used primarily in the recovery step of Escherichia coli competent cell transformations. The culture of E.coli cells in this nutrient-rich microbial broth increases the transformation efficiency of recombinant plasmids containing toxic protein genes by inhibiting the unintended activation of the lac promoter carried on the cloning vector. SOC medium contains peptides, amino acids, water-soluble vitamins, and glucose in a low-salt formulation.

Application

SOC Medium has been used for the transformation of DH5α competent E-coli cells. It has also been used to culture white colonies post-cloning for the identification of the recombinant protein of interest.

Caractéristiques et avantages

SOC liquid provides:

• Filter-sterilized, ready-to-use format
• Convenient package sizes
• Standard formulation

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable