SA20

Starch (HK) Assay Kit

sufficient for 20 assays

• Synonyme(s) : Starch Quantitation Kit
• Code UNSPSC : 12164500
• Nomenclature NACRES : NA.84

Propriétés

• Utilisation: sufficient for 20 assays
• Niveau de qualité: 100
• Classe(s) chimique(s) de l'analyte: sugars
• Technique(s): photometry: suitable
• Application(s): food and beverages; general analytical
• Température de stockage: 2-8°C

Description

Application

This kit is for the quantitative, enzymatic determination of native starch in food and other materials using the amyloglucosidase-hexokinase-glucose 6-phosphate dehydrogenase enzymatic assay. The hydrolysis of starch to glucose is catalyzed by amyloglucosidase. Glucose is phosphorylated by adenosine triphosphate (ATP) in the reaction catalyzed by hexokinase. Glucose-6-phosphate (G6P) is then oxidized to 6-phosphogluconate in the presence of nicotinamide adenine dinucleotide (NAD) in a reaction catalyzed by glucose-6-phosphate dehydrogenase (G6PDH). During this oxidation, an equimolar amount of NAD is reduced to NADH. The increase in absorbance at 340 nm is directly proportional to the glucose concentration.

Composants de kit seuls

• glucose assay reagent 20 mL
• starch assay reagent 1 mL/vial
• starch assay standard 5 g

Informations sur la sécurité

• Pictogrammes: GHS07
• Mention d'avertissement: Warning
• Mentions de danger: H315,H319,H335
• Conseils de prudence: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• Classification des risques: Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
• Organes cibles: Respiratory system
• Code de la classe de stockage: 11 - Combustible Solids