SRP3039

FGF-23 human

recombinant, expressed in E. coli, ≥95% (SDS-PAGE), ≥95% (HPLC), suitable for cell culture

• Synonyme(s) : Fibroblast Growth Factor-23
• Code UNSPSC : 12352202
• Nomenclature NACRES : NA.32

Propriétés

• Source biologique: human
• Produit recombinant: expressed in E. coli
• Essai: ≥95% (HPLC); ≥95% (SDS-PAGE)
• Forme: lyophilized
• Poids mol.: 25.5 kDa
• Conditionnement: pkg of 20 μg
• Technique(s): cell culture | mammalian: suitable
• Impuretés: <0.1 EU/μg endotoxin, tested
• Couleur: white to off-white
• Numéro d'accès UniProt: Q9GZV9

Description

Description générale

Fibroblast growth factor-23 (FGF-23) is a bone-derived hormone which is secreted by osteocytes and osteoblasts. It is part of the atypical FGF subfamily. The gene encoding it is localized on human chromosome 12p13.[1][2] Recombinant human FGF-23 is a 25.5kDa globular protein containing 228 amino acid residues.

Actions biochimiques/physiologiques

The FGF family plays a central role during prenatal development and postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. Fibroblast growth factor-23, -21 and -19 (FGF-23, FGF-21 and FGF-19) act as circulating hormones and require the participation of a Klotho protein as a co-receptor for their signaling. The signaling receptor for FGF-23, a Klotho-FGFR1 (IIIc) complex,[1] is an essential regulator of the renal sodium phosphate co-transporter and key vitamin D-metabolizing enzymes cytochrome P450 family 27 subfamily B member 1 (CYP27B1)[3] and cytochrome P450 family 24 subfamily A member 1 (CYP24A1).[4] FGF-23 acts in the kidney to regulate phosphate homeostasis and vitamin D metabolism.[4]

Séquence

MYPNASPLLG SSWGGLIHLY TATARNSYHL QIHKNGHVDG APHQTIYSAL MIRSEDAGFV VITGVMSRRY LCMDFRGNIF GSHYFDPENC RFQHQTLENG YDVYHSPQYH FLVSLGRAKR AFLPGMNPPP YSQFLSRRNE IPLIHFNTPI PRRHTRSAED DSERDPLNVL KPRARMTPAP ASCSQELPSA EDNSPMASDP LGVVRGGRVN THAGGTGPEG CRPFAKFI

Forme physique

Lyophilized from 10 mM Sodium Acetate, pH 6.0 + 0.1 M Arginine.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.5 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable