T2507

Triosephosphate Isomerase from baker′s yeast (S. cerevisiae)

Type I, ammonium sulfate suspension, ~10,000 units/mg protein

• Synonyme(s) : D-Glyceraldehyde-3-phosphate ketol-isomerase, TPI
• CAS Number: 9023-78-3
• Numéro CE : 5.3.1.1 (BRENDA, IUBMB)
• Numéro CE : 232-774-2
• Numéro MDL: MFCD00082080
• Code UNSPSC : 12352204
• Nomenclature NACRES : NA.54

Propriétés

• Type: Type I
• Niveau de qualité: 200
• Forme: ammonium sulfate suspension
• Activité spécifique: ~10,000 units/mg protein
• Poids mol.: 53 kDa by equilibrium centrifugation
• Activité étrangère: pyruvate kinase, lactic dehydrogenase, 3-phosphoglyceric phosphokinase, phosphoglucose isomerase, α-glycerophosphate dehydrogenase, aldolase, and glyceraldehyde-3-phosphate dehydrogenase <0.01%
• Température de stockage: 2-8°C

Description

Application

Triosephosphate isomerase has been used in a study to assess differential expression of fourteen proteins of uveal melanoma. Triosephosphate isomerase has also been used in a study to investigate the use of sigmoid pH gradients in free-flow isoelectric focusing of human endothelial cell proteins.

Définition de l'unité

One unit will convert 1.0 μmole D-glyceraldehyde 3-phosphate to dihydroxyacetone phosphate per min at pH 7.6 at 25 °C.

Forme physique

Crystalline suspension in 2.7 M (NH₄)₂SO₄, 0.5 mM EDTA, pH 6.5

Remarque sur l'analyse

Protein determined by biuret.

Autres remarques

Since the assay of this enzyme seems to be sensitive to minor variables, packages are dispensed per mg protein rather than in units.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)