T3573

RANK Ligand/TRANCE human

>90% (SDS-PAGE), recombinant, expressed in NSO cells, lyophilized powder

• Synonyme(s) : Osteoclast Differentiation Factor (ODF), Osteoprotegerin Ligand (OPGL), Receptor Activator of NF-KB Ligand (RANKL), TNF-related activation-induced cytokines (TRANCE)
• CAS Number: 207621-35-0
• Numéro MDL: MFCD01867142
• Code UNSPSC : 12352202
• Nomenclature NACRES : NA.32

Propriétés

• Source biologique: human
• Niveau de qualité: 200
• Produit recombinant: expressed in NSO cells
• Essai: >90% (SDS-PAGE)
• Forme: lyophilized powder
• Puissance: 1.5-7.5 ng/mL ED₅₀
• Poids mol.: glycolysylated recombinant product ~35 kDa by SDS-PAGE; calculated mol wt 23 kDa
• Conditionnement: pkg of 10 μg
• Conditions de stockage: avoid repeated freeze/thaw cycles
• Impuretés: endotoxin, tested
• Numéro d'accès UniProt: O14788
• Application(s): cell analysis
• Température de stockage: −20°C
• Informations sur le gène: human ... TNFSF11(8600)

Description

Description générale

Tumor necrosis factor (ligand) superfamily, member 11 (Tnfsf11) is also known as receptor activator of nuclear factor-κB ligand (RANKL). It is a type II transmembrane signaling receptor. It has a molecular weight of around 35kDa. This protein contains an amino-terminal intracellular tail and a carboxy-terminal extracellular region, that bears a connecting stalk and a receptor-binding domain. Tnfsf11 is located on human chromosome 13q14.11.

Application

RANK Ligand/TRANCE human can be used in in vitro osteoclastogenic assays for osteoclast differentiation.

RANK Ligand/TRANCE human has been used to stimulate differentiation.

Actions biochimiques/physiologiques

Member of the TNF superfamily; induces activation of the c-jun N-terminal kinase, enhances T-cell growth and dendritic cell function, induces osteoclastogenesis, and lymph node organogenesis; cell surface receptor for RANK Ligand.

RANK Ligand (receptor activator of NF-kB ligand (RANKL) also referred to as TNF-related activation induced cytokines (TRANCE) or osteoprotegerin ligand is a protein that belongs to tumor necrosis factor (TNF) family. It stimulates the mature dendritic cells and induces the cytokine production. RANKL also facilitates bone remodelling and possess an angiogenic activity.

RANK Ligand/TRANCE, a member of the TNF superfamily, induces activation of the c-jun N-terminal kinase, enhances T-cell growth and dendritic cell function, induces osteoclastogenesis, and lymph node organogenesis. Mouse and human RANK Ligand share 85% amino acid identity. RANK is the cell surface receptor for RANK Ligand.

Forme physique

Lyophilized from a 0.2 μm filtered solution in 20 mM MOPS and 500 mM NaCl, pH 6.5, with 50 μg BSA per 1 μg as a carrier protein.

Remarque sur l'analyse

The biological activity is measured by its ability to induce osteoclast differentiation on mouse splenocytes.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)