T7915
Thioredoxin Reductase from Escherichia coli
ammonium sulfate suspension, >25 units/mg protein (Bradford)
Synonyme(s) :
NADPH:oxidized thioredoxin oxidoreductase
Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
About This Item
CAS Number:
Numéro MDL:
Code UNSPSC :
12352204
Nomenclature NACRES :
NA.32
Produits recommandés
Source biologique
Escherichia coli
Niveau de qualité
Forme
ammonium sulfate suspension
Activité spécifique
>25 units/mg protein (Bradford)
Poids mol.
70 kDa
Technique(s)
activity assay: suitable
Numéro d'accès UniProt
Température de stockage
2-8°C
Informations sur le gène
Escherichia coli K12 ... trxB(949054)
Description générale
Research area: Cell Signaling
Thioredoxin reductases (TrxRs) belongs to family of selenium-containing pyridine nucleotide-disulphide oxidoreductases.
Thioredoxin reductases (TrxRs) belongs to family of selenium-containing pyridine nucleotide-disulphide oxidoreductases.
Application
Thioredoxin Reductase from Escherichia coli can be used in peroxidase-coupled thioredoxin system assay for assessing the peroxidase activitiy of Cys-based thiol peroxidases.Thioredoxin Reductase from Escherichia coli has been used:
- for determining the enzymatic activity of His6-Ahp1p.
- to assay C. elegans TRXR using the TXN-dependent activity assay.
- to investigate the biological reducing systems for organic hydroperoxide resistance R gene (OhrR).
- for enzymatic targeting of auranofin to test its antimicrobial properties.
- in thioredoxin reductase activity and peroxiredoxin assay.
- to study the synergy between broccoli sprout extract and selenium in the upregulation of thioredoxin reductase in human hepatocytes.
Thioredoxin reductase from Escherichia coli has been used in thioredoxin reductase activity and peroxiredoxin assay.
It has also been used to study the synergy between broccoli sprout extract and selenium in the upregulation of thioredoxin reductase in human hepatocytes.
It has also been used to study the synergy between broccoli sprout extract and selenium in the upregulation of thioredoxin reductase in human hepatocytes.
Actions biochimiques/physiologiques
An FAD-containing enzyme involved in the transfer of hydrogen from E. coli thioredoxin to other proteins thus providing a powerful disulfide reductase system.
Thioredoxin reductase (TrxR) is an NADPH-dependent oxidoreductase containing one FAD per subunit that reduces the active site disulfide in oxidised thioredoxin (Trx). The molecular weight of the isozymes from mammalian sources vary between 55-67 kDa as compared with 35 kDa in prokaryotes, plants or yeast. The substrate specificity of the mammalian enzyme is much broader than the prokaryotic enzyme reducing both mammalian and E. coli thioredoxins as well as well as non-disulfide substrates such selenite, lipoic acids, lipid hydroperoxides and hydrogen peroxide.
Thioredoxin reductase is a FAD containing enzyme, which transfers the reducing equivalent from NADPH to the disulphide bond of the enzyme by using FAD moiety within the Cys-Ala-Thr-Cys sequence. It can also reduce Trx-S2 to Trx-(SH)2 by using NADPH.
Définition de l'unité
One unit will cause an increase in absorbance of 1.0 at 412 nm (when measured in a coupled assay with E. coli thioredoxin and DTNB) per min per mL at pH 7.0 at 25 °C.
Forme physique
Suspension in 3.6 M (NH4)2SO4 containing 30 mM potassium phosphate buffer, pH 7.5, and 2 mM EDTA.
Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Faites votre choix parmi les versions les plus récentes :
Déjà en possession de ce produit ?
Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..
Contacter notre Service technique