U0251

Urease from Canavalia ensiformis (Jack bean)

Type C-3, powder, ≥600,000 units/g solid

• Synonyme(s) : Jack bean urease, Urea amidohydrolase
• CAS Number: 9002-13-5
• Numéro CE : 3.5.1.5 (BRENDA, IUBMB)
• Numéro CE : 232-656-0
• Numéro MDL: MFCD00070858
• Code UNSPSC : 12352204
• eCl@ss : 32160410
• Nomenclature NACRES : NA.54

Propriétés

• Source biologique: Canavalia ensiformis
• Niveau de qualité: 200
• Type: Type C-3
• Forme: powder
• Activité spécifique: ≥600,000 units/g solid
• Poids mol.: ~544620 Da
• Produit purifié par: crystallization
• Température de stockage: −20°C

Description

Description générale

Subunit molecular weight: ~90,770
Composed of six subunits with total molecular weight: ~544,620

Urease is a nickel-dependent metalloenzyme , found in various plants, bacteria, fungi, and algae. It contains two distinct subunits-UreA(26.5 kDa) and UreB (60.3–61.6 kDa).

Actions biochimiques/physiologiques

Urease aids in the bioavailability of nitrogen in plants. It is implicated in defense mechanism and exhibits insecticidal activity. In addition, urease exhibits antifungal activity by retarding growth and affecting membrane integrity of filamentous fungi and yeasts.

Urease was shown to induce vacuolation in Hela cells, an effect dependent on the ability of urease to convert urea to ammonia.

Définition de l'unité

One micromolar unit will liberate 1.0 μmole of NH₃ from urea per min at pH 7.0 at 25 °C. It is equivalent to 1.0 I.U. or 0.054 Sumner unit (1.0 mg ammonia nitrogen in 5 minutes at pH 7.0 at 20 °C)

Informations sur la sécurité

• Pictogrammes: GHS08,GHS07
• Mention d'avertissement: Danger
• Mentions de danger: H315,H319,H334,H335
• Conseils de prudence: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• Classification des risques: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• Organes cibles: Respiratory system
• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 1