U7752

Urease from Canavalia ensiformis (Jack bean)

• CAS Number: 9002-13-5
• Numéro CE : 3.5.1.5 (BRENDA, IUBMB)
• Numéro CE : 232-656-0
• Numéro MDL: MFCD00070858
• Code UNSPSC : 12352204
• eCl@ss : 32160410
• Nomenclature NACRES : NA.54

Propriétés

• Source biologique: Canavalia ensiformis
• Niveau de qualité: 200
• Forme: solid
• Poids mol.: hexamer 545 kDa; trimer ~272 kDa
• Contient: ~20% dithiothreitol
• Adéquation: suitable for marker for electrophoresis (non-denaturing PAGE)
• Température de stockage: −20°C

Description

Description générale

Subunit molecular weight: ~90,770
Composed of six subunits with total molecular weight: ~544,620

Urease is a multi-subunit nickel-dependent metalloenzyme. It is found abundantly in fungi, yeast, plants, and bacteria. It belongs to the superfamily of amidohydrolases and phosphotriesterases. Urease is also found in leguminous seeds.

Application

Urease from Canavalia ensiformis (Jack bean) has been used:

• as a component of the molecular mass standard in electrophoresis
• as a component of the standard in size-exclusion chromatography
• as a reference in native-polyacrylamide gel electrophoresis (PAGE)

Actions biochimiques/physiologiques

Urease is an essential enzyme of the nitrogen cycle. It plays a role in degrading urea into ammonia and carbamate. Urease provides a source of nitrogen for growth to various organisms. It also plays a major role in seed germination and seed chemical defense.

Autres remarques

Contains approx. 1 mg protein

Informations sur la sécurité

• Pictogrammes: GHS08,GHS07
• Mention d'avertissement: Danger
• Mentions de danger: H315,H319,H334,H335
• Conseils de prudence: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• Classification des risques: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• Organes cibles: Respiratory system
• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 1