U3383

Urease Buffer Reagent

• Synonyme(s) : Urea colorimetric determination assay reagent
• Code UNSPSC : 12352204
• Nomenclature NACRES : NA.54

Propriétés

• Description: When reconstituted, product is a solution of urease from Jack bean in 27 mM EDTA with 0.1% sodium azide as a preservative.
• Niveau de qualité: 200
• Forme: powder
• Poids mol.: 545 kDa
• Température de stockage: 2-8°C

Description

Description générale

Urease is a 545 kDa protein critical for the hydrolysis of urea to carbon dioxide and ammonia.

Urease is a dinickel enzyme that hydrolyses urea into ammonia and carbon dioxide. It is secreted by several bacterial species, invertebrates, fungi, and plants. Detection of urease activity is a potential diagnostic tool for Helicobacter pylori infections and several oral bacterial infections. two Ni²⁺ ions bridged by a hydroxyl group and a carbamylated lysine.

Application

Urease Buffer Reagent has been used:

• to measure plasma urea N (PUN) by the hypochlorite-phenol nitroprusside method
• in cell viability assay to measure the synthesis of urea in human fetal stem/progenitor cells
• to study the metabolic capacity of rat hepatocytes

Used in conjunction with Phenol Nitroprusside solution (Product Code P 6994) and Alkaline Hydpochlorite solution (Product Code A 1727) for the colorimetric determination of urea.

Informations sur la sécurité

• Pictogrammes: GHS08
• Mention d'avertissement: Danger
• Mentions de danger: H315 - H319 - H334
• Conseils de prudence: P261 - P264 - P280 - P284 - P302 + P352 - P305 + P351 + P338
• Classification des risques: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2
• Code de la classe de stockage: 13 - Non Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter