V3638

Vascular Endothelial Growth Factor 164 from rat

>95% (SDS-PAGE), recombinant, expressed in NSO cells, lyophilized powder

• Synonyme(s) : VEGF 164, VEGF-164, VEGF₁₆₄, Vasculotropin
• Numéro MDL: MFCD01636149
• Code UNSPSC : 12352202
• Nomenclature NACRES : NA.32

Propriétés

• Product Name: Vascular Endothelial Growth Factor 164 from rat, >95% (SDS-PAGE), recombinant, expressed in NSO cells, lyophilized powder
• Source biologique: rat
• Niveau de qualité: 200
• Produit recombinant: expressed in NSO cells
• Essai: >95% (SDS-PAGE)
• Forme: lyophilized powder
• Puissance: 1.08 ng/mL ED₅₀
• Poids mol.: dimer 50 kDa by SDS-PAGE; monomer 25 kDa by SDS-PAGE
• Conditionnement: pkg of 10 μg
• Conditions de stockage: avoid repeated freeze/thaw cycles
• Impuretés: endotoxin, tested
• Numéro d'accès UniProt: P16612
• Température de stockage: −20°C
• Informations sur le gène: rat ... Vegfa(83785)

Description

Application

Vascular Endothelial Growth Factor 164 (VEGF 164) from rat was used for cardiomyocytes culture and Ca²⁺ transients′ measurement. It was used as a medium M-199 supplement utilized in in vitro incubation for examining the capability of CTS to limit the impact of biologically relevant molecules, in particular epidermal growth factor (EGF), basic fibroblast growth factor (FGF2), and vascular endothelial growth factor A (VEGFA) in rat epididymis segment 1 and 2.

Actions biochimiques/physiologiques

Mitogen for endothelial cells, enhances vascular permeability, activates, and chemoattracts monocytes and is a procoagulant.

Vascular Endothelial Growth Factor 164 also referred to as VEGF 164 or Vasculotropin is a growth factor that induces endothelial cell growth, vasculogenesis and angiogenesis. VEGF 164 can enhance collagen and fibronectin expression in mesangial cells which in turn stimulates TGF-β1-induced extracellular matrix. It also plays a pivotal role in progressive glomerular fibrosis.

Autres remarques

164 amino acid variant of rat VEGF.

Forme physique

Lyophilized from a 0.2 μm filtered solution in 35% acetonitrile and 0.1% TFA containing 0.5 mg bovine serum albumin.

Remarque sur l'analyse

The biological activity is measured by its ability to stimulate ³H-thymidine incorporation in human umbilical vein endothelial cells.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)