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MABC128

Sigma-Aldrich

Anti-FIP200 Antibody, clone 14E11.2

clone 14E11.2, from mouse

Synonym(s):

RB1-inducible coiled-coil protein, 1FAK family kinase-interacting protein of 200 kDa, FIP200

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100 MG
MYR 689.00
1 G
MYR 1,746.00

MYR 689.00

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100 MG
MYR 689.00
1 G
MYR 1,746.00

About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

MYR 689.00

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biological source

mouse

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

14E11.2, monoclonal

species reactivity

human, mouse

technique(s)

immunocytochemistry: suitable
western blot: suitable

isotype

IgG2bκ

NCBI accession no.

NP_001077086

UniProt accession no.

Q8TDY2

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Anti-TTI1 affinity isolated antibody

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Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

antibody form

affinity isolated antibody

antibody form

purified immunoglobulin

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

biological source

rabbit

biological source

mouse

biological source

rabbit

biological source

rabbit

technique(s)

immunohistochemistry: 1:25- 1:100

technique(s)

capture ELISA: suitable, immunofluorescence: suitable, indirect ELISA: suitable, western blot: 1-5 μg/mL

technique(s)

immunoblotting: 0.04-0.4 μg/mL, immunofluorescence: 0.25-2 μg/mL, immunohistochemistry: 1:50-1:200

technique(s)

immunohistochemistry: 1:25-1:100

Gene Information

human ... MT2A(4502)

Gene Information

human ... MT2A(4502)

Gene Information

human ... MTA2(9219)

Gene Information

human ... TTI1(9675)

UniProt accession no.

P02795

UniProt accession no.

P02795

UniProt accession no.

O94776

UniProt accession no.

O43156

General description

Focal adhesion kinase family interacting protein of 200 kDa (FIP200) is a novel protein inhibitor for focal adhesion kinase (FAK). FIP200 inhibits kinase activity and associated cellular functions, such as cell adhesion, spreading, and motility in fibroblasts by binding directly to FAK.

The previously assigned protein identifier Q86YR4 has been merged into Q8TDY2. Full details can be found on the UniProt database.

Specificity

This antibody binds to the interaction domain of FIP200, which is needed for the formation of the ULK-FIP200 complex under starved, autophagy conditions.

Immunogen

Epitope: Interaction domain
GST-tagged recombinant protein corresponding to the interaction domain of human FIP200.

Application

Anti-FIP200 Antibody, clone 14E11.2 is an antibody against FIP200 for use in Western Blotting, ICC.
Research Category
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional
Western Blot Analysis: 1 µg/mL from a representative lot detected FIP200 in serum starved and non-serum starved HeLa cell lysate.

Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected FIP200 in NIH/3T3, A431, and HeLa cells.

Immunocytochemistry Analysis: A 1:250 dilution from a representative lot detected FIP200 in untreated and chloroquinone-treated HUVEC cells.

Quality

Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected FIP200 in 10 µg of HeLa cell lysate.

Target description

~200 kDa observed. An uncharacterized band may be observed at ~50 kDa in some cell lysates.

Physical form

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
HeLa cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

  • Specification Sheet

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    M F Pardo et al.
    Journal of agricultural and food chemistry, 48(9), 3795-3800 (2000-09-20)
    A new plant endopeptidase was obtained from unripe fruits of Bromelia balansae Mez (Bromeliaceae). Crude extracts were partially purified by ethanol fractionation. This preparation (redissolved ethanol precipitate, REP) showed maximum activity at pH 8.8-9.2, was very stable even at high
    Paul V Attwood et al.
    Analytical biochemistry, 334(2), 382-389 (2004-10-21)
    Mass spectrometry offers a potential means of measuring virtually all enzyme-catalyzed reactions by simultaneously measuring the concentrations of substrates, products, and intermediates where there are differences in mass between them. To perform these measurements the reaction mixture must be aged
    M F Pardo et al.
    Biological chemistry, 382(5), 871-874 (2001-08-24)
    Unripe fruit extracts of Bromelia balansae Mez (Bromeliaceae), whose principal endopeptidase is balansain I (isolated for anion exchange chromatography: pI = 5.45, molecular weight = 23192), exhibit a pH profile with a maximum activity around pH 9.0 and are inhibited
    Susana R Morcelle et al.
    Fitoterapia, 75(5), 480-493 (2004-07-21)
    As part of a screening of latex endopeptidases from plants growing in Argentina, the presence of proteolytic activity in the latex of Funastrum clausum stems is reported. The proteases present in the crude extract showed the main characteristics of the
    N E Mackenzie et al.
    The Biochemical journal, 219(2), 437-444 (1984-04-15)
    The kinetics of the trypsin-catalysed hydrolysis of the highly specific substrate N alpha-benzyloxycarbonyl-L-lysine p-nitrophenyl ester were studied under cryoenzymological conditions by 13C-n.m.r. spectroscopy at pH approx. 3.0. The kinetics of this reaction are shown to be in agreement with similar
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