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Key Documents

N4754

Sigma-Aldrich

Nigrosin water soluble

Powder

Synonym(s):

Acid black 2

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About This Item

CAS Number:
Colour Index Number:
50420
MDL number:
UNSPSC Code:
12171500
NACRES:
NA.47

Product Name

Nigrosin water soluble, For use as a biological stain

Quality Level

form

powder

solubility

water: 1 mg/mL, turbid

εmax

~250 at 575 nm in 50% ethanol

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

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General description

Nigrosin is an acidic dye used as a negative stain for visualization of the capsule in bacteriological material.

Application

Nigrosin has the following applications:
  • to stain live sperm
  • as a viable cell staining agent especially in stem cell biology
  • as a negative stain in place of India ink for spirochetes, bacteria, protozoa, and fungi
  • in Dorner′s spore stain.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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