68957

Tris(2-carboxyethyl)phosphine hydrochloride

BioUltra, suitable for electrophoresis, SDS-PAGE tested

• Synonym(s): TCEP.HCL, TCEP
• Empirical Formula (Hill Notation): C₉H₁₅O₆P · HCl
• CAS Number: 51805-45-9
• Molecular Weight: 286.65
• Beilstein: 3724376
• MDL number: MFCD00145469
• UNSPSC Code: 12352128
• PubChem Substance ID: 329761275
• NACRES: NA.32

Properties

• product line: BioUltra
• Quality Level: 100
• Assay: 97.5-102.5%
• form: powder
• technique(s): electrophoresis: suitable
• solubility: H₂O: soluble
• suitability: SDS-PAGE tested
• SMILES string: Cl[H].OC(=O)CCP(CCC(O)=O)CCC(O)=O
• InChI: 1S/C9H15O6P.ClH/c10-7(11)1-4-16(5-2-8(12)13)6-3-9(14)15;/h1-6H2,(H,10,11)(H,12,13)(H,14,15);1H
• InChI key: PBVAJRFEEOIAGW-UHFFFAOYSA-N

Description

General description

Tris(2-carboxyethyl) phosphine hydrochloride (TCEP.HCL ) is a useful reducing agent for Sulphur and nitrogen-containing compounds. TCEP.HCL and the resultant oxide are water-soluble. TCEP.HCL has electron-rich phosphorus and oxygen atoms to combine with various metals, including Rh, Ir, and Pd. It rapidly complexes with Rh and Ir in dioxane at ambient temperature. It reduces disulfide linkages in a range of peptides and proteins in biological disulfide.

Application

TCEP.HCL has been used in various bioconjugation applications, both in vitro and in vivo, to tag proteins and live organisms with great effectiveness under mild physiological circumstances. TCEP.HCL is suitable for varied biological and synthetic applications. At an acidic pH, TCEP.HCL can be used to convert organic disulfides to thiols in water irreversibly.

Water soluble reagent, used for selective reduction of disulfides. More stable than DTT and useful in mass spectrometry applications.

Features and Benefits

The product has the following benefits:

• TCEP.HCL lowers reactive oxygen species levels.
• It increases glutathione levels and mitochondrial content.
• It has anti-cancer effects.

Analysis Note

agarose gel electrophorese corresponds to requirements

Safety Information

• Pictograms: GHS05
• Signal Word: Danger
• Hazard Statements: H314
• Precautionary Statements: P260 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 - P363
• Hazard Classifications: Eye Dam. 1 - Skin Corr. 1B
• Storage Class Code: 8A - Combustible corrosive hazardous materials
• WGK: WGK 1