96669

7-Diethylamino-3-(4-maleimidophenyl)-4-methylcoumarin

suitable for fluorescence, BioReagent, ≥90% (HPCE)

• Synonym(s): 1H-Pyrrole-2,5-dione, 1-(4-(7-(diethylamino)-4-methyl-2-oxo-2H-1-benzopyran-3-yl)phenyl)-, CPM
• Empirical Formula (Hill Notation): C₂₄H₂₂N₂O₄
• CAS Number: 76877-33-3
• Molecular Weight: 402.44
• MDL number: MFCD00077334
• UNSPSC Code: 12171500
• PubChem Substance ID: 57653774
• NACRES: NA.32

Properties

• product line: BioReagent
• Quality Level: 100
• Assay: ≥90% (HPCE)
• form: solid
• fluorescence: λ_ex 391 nm; λ_em 472 nm in methanol: 2-mercaptoethanol
• suitability: suitable for fluorescence
• storage temp.: −20°C
• SMILES string: CCN(CC)c1ccc2C(C)=C(C(=O)Oc2c1)c3ccc(cc3)N4C(=O)C=CC4=O
• InChI: 1S/C24H22N2O4/c1-4-25(5-2)18-10-11-19-15(3)23(24(29)30-20(19)14-18)16-6-8-17(9-7-16)26-21(27)12-13-22(26)28/h6-14H,4-5H2,1-3H3
• InChI key: YGIABALXNBVHBX-UHFFFAOYSA-N

Description

Application

Fluorescence probe for glutathione for intact cells; specific advantages: only thio derivative fluoresces, high quantum yield and good Stokes shift

Biochem/physiol Actions

Used to monitor release of thiols, quantitate thiol in microplate reactions, and to distinguish proliferating cancer cells by nucleolar protein staining.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves