A1908

Asialofetuin from fetal calf serum

Type II (Sigma designation)

• MDL number: MFCD00130561
• UNSPSC Code: 12352202
• NACRES: NA.61

Properties

• biological source: bovine
• type: Type II (Sigma designation)
• form: powder
• technique(s): activity assay: suitable; inhibition assay: suitable
• impurities: salt, essentially free; ≤1.0% N-acetylneuraminic acid
• solubility: H₂O: soluble 1.0 mg/mL, clear to hazy, colorless to very faintly yellow
• storage temp.: 2-8°C

Description

Application

Asialofetuin from fetal calf serum is suitable for use:

• in the assay of the lactose binding activity of the galectin-7 by immunodetection of in situ binding activity to asialofetuin immobilized on nitrocellulose
• in the inhibition of zona pellucida hardening of mouse oocytes
• as an acceptor in sialyltransferase enzyme activity assay

Incorporation of asialofetuin (AF) in liposomes strongly enhance delivery to and endocytosis by cells displaying the AF receptor, notably hepatocytes. This provides a very efficient route for gene therapy.

Biochem/physiol Actions

Asialofetuin (AF) binds to asialoglycoprotein receptor (ASGP-R) and is employed as a ligand for drug delivery to the hepatocytes. It aids serum-resistant lipoplexes for efficient gene deliver into the HepG2 (human hepatoblastoma) cells.

Feutins are glycoproteins that belong to the cystatin superfamily. They are characterized by the presence of two tandemly arranged cystatin-like domains and an unrelated domain. It binds to transforming growth factor (TGF)-β/BMP (bone morphogenic protein) cytokines.

Preparation Note

Enzymatic cleavage using neuraminidase

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves