A4781

Asialofetuin from fetal calf serum

Type I (Sigma designation)

• Synonym(s): Asialofetuin
• MDL number: MFCD00130561
• UNSPSC Code: 12352202
• NACRES: NA.61

Properties

• biological source: bovine (calf) serum
• Quality Level: 300
• type: Type I (Sigma designation)
• form: powder
• impurities: salt, essentially free; ≤0.5% N-acetylneuraminic acid
• solubility: 0.85% sodium chloride: soluble 1 mg/mL
• storage temp.: 2-8°C

Description

General description

Asialofetuin is a glycoprotein with three asparagine-linked triantennary complex carbohydrate chains and terminal N-acetylgalactosamine residues.

Application

Asialofetuin from fetal calf serum has been used:

• to quantitate the plant Ricin toxin′s B subunit (RTB) lectin activity in β-phaseolin signal peptide (P)–proinsulin gene (INS)–RTB plants by enzyme-linked immunosorbent assay (ELISA)
• quantitate VP7:RTB fusion protein in transformed potato tissues by ELISA
• as a glycoprotein substrate to measure the receptor-binding activity of recombinant RTB and NSP490–RTB fusion proteins
• to study the nature of the interaction between ferritin and the placenta

Incorporation of asialofetuin (AF) in liposomes strongly enhance delivery to and endocytosis by cells displaying the AF receptor, notably hepatocytes. This provides a very efficient route for gene therapy.

Biochem/physiol Actions

Asialofetuin exhibits affinity to asialoglycoprotein receptor (ASGP-R) on hepatocytes and uses the receptor to enter the cell. This property allows asialofetuin to be used as a ligand to deliver drugs to hepatocytes and as a competitive inhibitor to ASGP-R.

Preparation Note

Prepared by a modification of Spiro, R.G., J. Biol. Chem., 235, 2860 (1960).

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves