C2273

Carbonic Anhydrase from bovine erythrocytes

For use as a marker in SDS-PAGE

• Synonym(s): Carbonate Dehydratase, Carbonate Hydrolyase
• CAS Number: 9001-03-0
• EC Number: 4.2.1.1 (BRENDA, IUBMB)
• EC Number: 232-576-6
• MDL number: MFCD00081467
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• biological source: bovine erythrocytes
• Quality Level: 200
• form: powder
• mol wt: ~29 kDa
• packaging: vial of 5 mg
• storage temp.: 2-8°C

Description

General description

Carbonic Anhydrase is a metalloenzyme with zinc. The active site of carbonic anhydrase contains three conserved histidine residues (His94, His96 and His119).

Application

Carbonic Anhydrase has been used:

• as a component of nine-protein mixture for the comparative proteomic mass spectrometry analysis of bovine brain samples
• as a molecular marker in gel filtration chromatography
• as a standard protein to test the recovery of CMA/20 microdialysis probe

Biochem/physiol Actions

Carbonic anhydrase is essential for the reversible hydration of carbon dioxide. It is also crucial for the physiological processes including respiration, ion transport, acid–base balance, lipid and carbohydrate metabolic pathways. Carbonic anhydrase participates in electrolyte secretion and in the carbon dioxide fixation.

Unit Definition

One collagen digestion unit (CDU) liberates peptides from collagen from bovine achilles tendon equivalent in ninhydrin color to 1.0 μmole of leucine in 5 hours at pH 7.4 at 37 °C.

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Statements: H334
• Precautionary Statements: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves