E6754

Epoxy-activated−Sepharose^™ 6B

lyophilized powder

• Synonym(s): Epoxy-activated-Agarose
• MDL number: MFCD00163561
• UNSPSC Code: 41106500
• NACRES: NA.56

Properties

• form: lyophilized powder
• Quality Level: 200
• extent of labeling: 19-40 μmol per mL
• technique(s): affinity chromatography: suitable
• matrix: Sepharose 6B
• matrix active group: epoxy
• matrix attachment: through epoxy to hydroxyl
• matrix spacer: 12 atoms
• swelling: 1 g swells to 3.0-5.0 mL
• particle size: 45—165 μm
• storage temp.: 2-8°C

Description

General description

when ligands are coupled to free epoxy groups. Linkage is a stable, uncharged ether.

Application

Epoxy-activated-Sepharose^™ is used in affinity chromatography, protein chromatography, and activated/functionalized matrices. Epoxy-activated-Sepharose^™ has been used to obtain a monoclonal antibody against the prokaryotically expressed foot-and-mouth disease virus (FMDV) non-structural protein (NSP) 3B as well as to study B meningococcal disease.

Other Notes

Ligand: 1,4-bis(2:3-Epoxypropoxy)butane
Attachment: one epoxy group
Spacer: provides a 12-atom spacer when ligands are coupled to free epoxy groups. Linkage is a stable, uncharged ether.

Physical form

Lyophilized powder

Legal Information

Sepharose is a trademark of Cytiva

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves