G9270

γ-Glutamyltranspeptidase from equine kidney

Type VI, 5-12 units/mg solid

• Synonym(s): γ-GT, γ-Glutamyltransferase, (5-Glutamyl)peptide:amino-acid 5-glutamyltransferase, GGTP
• CAS Number: 9046-27-9
• EC Number: 2.3.2.2 (BRENDA, IUBMB)
• EC Number: 232-931-5
• MDL number: MFCD00131126
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• biological source: equine kidney
• Quality Level: 200
• type: Type VI
• form: solid
• specific activity: 5-12 units/mg solid
• storage temp.: −20°C

Description

General description

Gamma-Glutamyltranspeptidase is a heterodimeric enzyme and belongs to the superfamily of N-terminal nucleophile hydrolases.

Application

Gamma-glutamyltranspeptidase from equine kidney has been used:

• to identify the formation of 4-S-Cysteinyltetrodotoxin in the liver of Fugu pardalis
• as a standard to determine the activity of gamma-glutamyltransferase from human B-cell lymphoma cell lysates
• to hydrolyze isopeptides to study its effects on tubulin aggregation

Biochem/physiol Actions

Gamma-Glutamyltranspeptidase participates in glutathione metabolism and it catalyzes the cleavage of gamma-glutamyl compounds, such as glutathione. It also plays a role in the transfer of gamma-glutamyl moiety to amino acids and peptides.

Quality

Crude

Unit Definition

One unit will liberate 1.0 μmole of p-nitroaniline from L-γ-glutamyl-p-nitroanilide per min at pH 8.5 at 25°C.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves