I2771

ITS+3 Liquid Media Supplement (100×)

liquid, sterile-filtered, BioReagent, suitable for cell culture

• Synonym(s): ITS Supplement
• UNSPSC Code: 12352205
• NACRES: NA.75

Properties

• sterility: sterile-filtered
• product line: BioReagent
• form: liquid
• technique(s): cell culture | mammalian: suitable
• impurities: endotoxin, tested
• shipped in: ambient
• storage temp.: 2-8°C

Description

General description

ITS+3 liquid media supplement is a mixture of insulin, human transferrin (partially iron-saturated), sodium selenite, linoleic acid, and bovine serum albumin (BSA). It is suitable for serum-free cell culture applications.

Application

ITS+3 Liquid Media Supplement (100×) has been used as a media supplement:

• to support the growth of human adipose stem cells (ASC)
• to culture porcine intervertebral disc (IVD) cells and tissues
• to culture cartilage endplate (CEP) cells

Biochem/physiol Actions

• Insulin: a polypeptide hormone that promotes glucose and amino acid uptake and may produce a mitogenic effect.
• Transferrin: an iron-transport protein. Iron is an essential trace element but can be toxic in its free form. To nourish cells in culture, iron is supplied bound to transferrin in serum.
• Selenium: an essential trace element present in serum.

Components

Contains 1.0 mg/ml bovine insulin, 0.55 mg/ml human transferrin (substantially iron-free), 0.5 μg/ml sodium selenite, 470 μg/ml linoleic acid, 470 μg/ml oleic acid and 50 mg/ml bovine serum albumin at the 100x concentration. Contains 2 moles each of linoleic acid and oleic acid per mole of albumin.

Preparation Note

Prepared in Earle′s Balanced Salt Solution (EBSS) without phenol red.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves