L0288

Lipid Mixture 1, Chemically Defined

liquid, sterile-filtered, BioReagent, suitable for cell culture

• Synonym(s): lipid mixture
• UNSPSC Code: 12352211
• NACRES: NA.75

Properties

• sterility: sterile-filtered
• Quality Level: 500
• product line: BioReagent
• form: liquid
• technique(s): cell culture | mammalian: suitable
• impurities: ≤10 EU/mL endotoxin
• storage temp.: 2-8°C

Description

General description

Lipid mixture 1, Chemically defined is recommended for use in cell culture at 1 to 10 mL per liter of medium.

Application

Lipid Mixture 1, Chemically Defined has been used:

• as a component of bovine serum albumin (BSA) containing Bracket-Oliphant (BO) medium for the resuspension of sperm pellet
• as a supplement in spheroid media for culturing primary human subcutaneous stromal vascular fraction cells
• to prepare a cell steatosis model for culturing of mouse and human hepatocytes

Other Notes

Contains non-animal derived fatty acids (2 μg/ml arachidonic and 10 μg/ml each linoleic, linolenic, myristic, oleic, palmitic and stearic), 0.22 mg/ml cholesterol from New Zealand sheep′s wool, 2.2 mg/ml Tween-80, 70 μg/ml tocopherol acetate and 100 mg/ml Pluronic F-68 solubilized in cell culture water.
The final product contains 10% ethanol by volume.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable